Recombinant Pig CYP2C49 Protein CYP2C49-131P from Creative BioMart | Biocompare.com

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Recombinant Pig CYP2C49 Protein

Description

Recombinant pig CYP2C49 protein and pig CYP-reductase without tag were coexpressed in E. coli

Product Specs
ItemRecombinant Pig CYP2C49 Protein
CompanyCreative BioMart
Price Supplier Page View Company Product Page
Catalog NumberCYP2C49-131P
Source/Expression SystemE. coli
Molecule NameCYP2C49 cytochrome P450 2C49 [ Sus scrofa (pig) ]
SpeciesPig
ConcentrationP450 Concentration: 10.0 nmol/ml Protein Concentration: 15.5 mg/ml Specific P450 Content: 646 pmol/mg protein
ActivityCytochrome c Reductase Activity: 303 nmol/min/mg protein P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]) Activity: Tolbutamide 4-hydroxylase Linear with time up to: 60 min(Determined at 350 µM substrate, using a P450 concentration of 100 pmol/ml) Linear with P450 up to: 120 pmol/ml(Determined at 350 µM substrate, using an incubation time of 20 min) Vmax: 0.41 pmol/min/pmol CYP Km: 586 µM

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Creative BioMart

Creative BioMart

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