Description
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine- rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 114 amino acids as a prepeptide, and its hydrophobic N-terminal 20 amino acid signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 94 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. Recombinant mouse Resistin is is a 13.7 kDa protein containing 93 amino acids of the mouse Resistin and 31 additional amino acids - the signal sequence of phage fd (21 aa) and the C-terminal fusion of the Flag-tag (10 aa)