Description
CD14 (also known lipopolysaccharide [LPS] receptor) is expressed strongly on monocytes and macrophages and weakly on the surface of neutrophils. CD14 (1-201 a.a.) is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentrations as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.:The mouse CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection, the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The recombinant mouse CD-14 has been purified by His- tag with Talon (Clontech) and controlled by SDS page