Description
Pepsin is an endopeptidase. It hydrolyses proteins and peptides favorably adjacent to aromatic and dicarboxylic L-amino acid residues, preferentially phenylalanine and leucine but not next to valine, alanine, and glycine. Pepsin is not stable above pH 6. 0. F7m: 1. 0 mg pepsin (16 m Anson) immobilized on polyvinyl per CR-column (1 m Anson unit is equivalent to 1 µmole of Foline-positive amino acids calculated as tyrosine released from denaturated hemoglobin per minute at 37. 5°C at pH 2; this mAnson unit is equivalent to ca. 180 D-A280 units). Nr. 40 Storage buffer : 50 mM glycine, 10% (v/v) glycerol, pH 4. 0 Nr. 33 Reaction buffer: 20 mM Na acetate, pH 4. 7. Nr. 34 Washing buffer: 20 mM Na acetate, 1. 0 M NaCl, pH 4. 7