Native Bacillus licheniformis Protease NATE-0633 from Creative Enzymes

Product Specs
ItemNative Bacillus licheniformis Protease
CompanyCreative Enzymes
Price Supplier Page View Company Product Page
Catalog NumberNATE-0633
TypeEnzyme
ActivityType VIII, 7-15 units/mg solid; Type I, > 2.4 U/g
Research AreaEnzymes for Research & Diagnostic Use

Creative Enzymes

Phone: 1-631-562-8517
1-516-512-3133

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(1) Dysregulation of alternative splicing patterns in the ovaries of reproductively aged miceReproductionMarch 15, 2026Adnan T Alsamaraee, Vanessa L Correll, Julius O Nyalwidhe, Pavla Brachova, Nehemiah S Alvarezenzymatically dissociated in 100 µl of 10 mg/ml (in HBSS) Bacillus licheniformis protease (Creative Enzymes, NATE-0633) and DNase I. Tissues were agitated at 4 °C at speeds ranging from 600 to 1000 rpm for up to 1 hr, with

(2) Revealing heterogeneity and damage response in the adult human utricleNat CommunDecember 11, 2025Emilia Luca, Neke Ibeh, Ryosuke Yamamoto, Weisheng Liang, Dallas Bennett, Vincent Lin, Joseph Chen, Michael Lovett, Alain Dabdoubmix was added: 200 µL (10 mg/mL) Native Bacillus licheniformis Protease–Subtilisin, (Creative Enzymes NATE-0633); 1 µL (125 U/mL) Deoxyribonuclease I from bovine pancreas (DNase I - Sigma-Aldrich D5025-15KU); 1 µL

(3) Tissue-resident macrophages co-develop with myocardial tissue in human induced pluripotent stem cell-derived organoidsFrontiers in Cell and Developmental BiologyNovember 13, 2025Anna Frederike Rockel, Tobias Brunnbauer, Nicole Wagner, Brenda Gerull, Süleyman Ergün, Philipp WörsdörferThe dissociation solution consisted of 10 mg/mL Bacillus licheniformis protease (Creative Enzymes, NATE-0633) and 125 U/mL DNase I (Merck, DN25) in ice-cold DPBS (Sigma-Aldrich) supplemented with 5 mM CaCl2 (Carl

(4) Primordial germ cell specification and early developmental cell states in Pacific oysterBMC GenomicsOctober 23, 2025Mackenzie R. Gavery, Lauren E. Vandepas, Lauren M. Saunders, Brent Vadopalas, J. Adam Luckenbach, Cole Trapnell, Steven RobertsµL of 10 mg/mL protease (Native Bacillus licheniformis protease; Creative Enzymes, Catalog Number: NATE0633) in 0.3x ASW incubated at room temperature for 15 min with intermittent gentle pipetting with a P1000

(5) Controlling nephron precursor differentiation to generate proximal-biased kidney organoids with emerging maturityNature communicationsAugust 30, 2025Schnell J, Miao Z, Achieng M, Fausto CC, Koppitch K, Takhirov L, Wang V, De Kuyper F, Huang B, Schreiber M, et al.as previously described27 using 10 mg/mL Native Bacillus licheniformis Protease (Creative BioMart, NATE-0633), 2.5 mg/mL collagenase type IV (Worthington Biochemical, LS004186), and 50 µg/mL DNase1 (Worthington Biochemical

(6) Single Cell Profiling in the Sox10Dom Hirschsprung Mouse Implicates Hox Genes in Enteric Neuron Trajectory AllocationCellular and Molecular Gastroenterology and HepatologyJuly 21, 2025Justin A. Avila, Joseph T. Benthal, Jenny C. Schafer, David K. Flaherty, E.Michelle Southard-Smithwere dissociated in a 1-mL solution volume containing native Bacillus licheniformis (Creative Enzymes: NATE-0633) at a concentration of 5 mg/mL, diluted in Hanks’ Balanced Salt Solution (HBSS) (Gibco:14185-052), supplemented

(7) Protocol for constructing whole-genome libraries from mini-biopsies by using laser capture microdissectionSTAR protocolsDecember 20, 2024Xi Y, Zhang S.N411-01 Optimum cutting temperature compound,Sakura,NA Native Bacillus licheniformis,Creative Enzymes,Cat# NATE-0633 "1 M Tris-HCl, pH = 7.6",Rockland Immunochemicals,Cat# MB-003 "0.5 M EDTA, pH = 8.0",Thermo Fisher Scientific

(8) PTGER4 signaling regulates class IIa HDAC function and SPINK4 mRNA levels in rectal epithelial cellsCell Communication and SignalingOctober 13, 2024Murugadas Anbazhagan, Garima Sharma, Shanta Murthy, Sushma Chowdary Maddipatla, Vasantha L. Kolachala, Anne Dodd, Amanda Randunne, David J. Cutler, Subra Kugathasan, Jason D. Matthewsthen enzymatically digested on ice using Bacillus licheniformis protease (Creative Enzymes, cat. no. NATE-0633). Then, cells and debris were filtered before determining cell viability and numbers. For organoids,

(9) Unraveling the key role of chromatin structure in cancer development through epigenetic landscape characterization of oral cancerMolecular cancerSeptember 6, 2024Xue Y, Liu L, Zhang Y, He Y, Wang J, Ma Z, Li TJ, Zhang J, Huang Y, Gao YQ.containing 15 µg/µl native cold-active Bacillus licheniformis Protease (Creative Enzymes, Cat. No. NATE-0633). The lysis reaction was conducted at 6 °C for 1 h. The released genomic DNA was further tagmented by

(10) A single-cell atlas of the miracidium larva of Schistosoma mansoni reveals cell types, developmental pathways, and tissue architectureeLifeAugust 27, 2024Teresa Attenborough, Kate A Rawlinson, Carmen L Diaz Soria, Kirsty Ambridge, Geetha Sankaranarayanan, Jennie Graham, James A Cotton, Stephen R Doyle, Gabriel Rinaldi, Matthew Berrimanadded to the larvae. We reconstituted 250 mg of the enzyme Native Bacillus licheniformis Protease (NATE-0633, Creative Enzymes) (Adam et al., 2017) in 2.5 ml of HBSS−/− (to give a stock concentration of 100 mg/ml)

(11) Spinal cord injury regulates circular RNA expression in axonsFrontiers in Molecular NeuroscienceAugust 24, 2023Mustafa M. Siddiq, Carlos A. Toro, Nicholas P. Johnson, Jens Hansen, Yuguang Xiong, Wilfredo Mellado, Rosa E. Tolentino, Kaitlin Johnson, Gomathi Jayaraman, Zaara Suhail, et al.(Miltenyi Biotech 130-100-008). Cold active protease from Bacillus licheniformis (Creative Enzymes NATE0633) was added (10 mg/ml of this protease with 125 U/ml DNase in ice cold Miltenyi MACS Tissue Storage Solution;

(12) A single‐cell RNA labeling strategy for measuring stress response upon tissue dissociationMolecular Systems BiologyFebruary 10, 2023Anika Neuschulz, Olga Bakina, Victor Badillo‐Lisakowski, Pedro Olivares‐Chauvet, Thomas Conrad, Michael Gotthardt, Helmut Kettenmann, Jan Philipp Junkerin 200 μl psychrophilic protease mix (1× Native Bacillus licheniformis Protease (Creative Enzymes #NATE‐0633) in HBSS, 10 mM 4sU) and incubated on ice for 30 min (bulk only). Dissociation was aided by pipetting

(13) Single-cell genomic variation induced by mutational processes in cancerNatureDecember 1, 2022Funnell T, O'Flanagan CH, Williams MJ, McPherson A, McKinney S, Kabeer F, Lee H, Salehi S, Vázquez-García I, Shi H, et al.collagenase/hyaluronidase at 37 °C for 2 h for TNBC tumours or with cold active Bacillus lichenformis (Creative Enzymes NATE0633) in PBS supplemented with 5 mM CaCl2 and 125 U ml−1 DNAse for HGSC tumours, as described previously45

(14) A scalable organoid model of human autosomal dominant polycystic kidney disease for disease mechanism and drug discoveryCell stem cellJuly 7, 2022Tran T, Song CJ, Nguyen T, Cheng SY, McMahon JA, Yang R, Guo Q, Der B, Lindström NO, Lin DC, et al.organoids were dissociated using 7.5 mg/ml Bacillus licheniformis cold active protease (Creative Enzymes, NATE-0633) mixed with 10 mg/ml collagenase type 2 (Worthington, #LS00417) and 125 U/ml DNase I (Worthington, #LS002058)

(15) A combined human gastruloid model of cardiogenesis and neurogenesisiScienceJune 17, 2022Olmsted ZT, Paluh JL.Anti-Adherence Rinsing Solution,STEMCELL Technologies,Cat.No.: 07010 Bacillus Licheniformis protease,Creative Biomart,Cat.No.: NATE-0633 DNase I,Sigma-Aldrich,Cat.No.: 11284932001 CryoStor CS-10 for cryopreservation,BioLife Solutions,Cat.No.:

(16) Uncompensated mitochondrial oxidative stress underlies heart failure in an iPSC-derived model of congenital heart diseaseCell stem cellMay 5, 2022Xu X, Jin K, Bais AS, Zhu W, Yagi H, Feinstein TN, Nguyen PK, Criscione JD, Liu X, Beutner G, et al.disaggregated using cold active protease [10 mg/ml Bacillus Licheniformis protease; Creative Enzymes NATE0633) and 125 U/ml DNase (Applichem, A3778) incubated on ice with trituration 5–7 minutes, then 5% bovine

(17) A type I interferon response defines a conserved microglial state required for effective neuronal phagocytosisbioRxiv : the preprint server for biologyFebruary 22, 2022Dorman LC, Nguyen PT, Escoubas CC, Vainchtein ID, Xiao Y, Lidsky PV, Nakajo H, Silva NJ, Lagares-Linares C, Wang EY, et al.psychrophilic proteases (PBS with 4 uL/mL DNAse (Sigma D4527–40KU), 5 mg/mL protease (Creative Enzymes NATE-0633, 175 ug/mL L-cysteine) incubated on ice for 20 minutes with gentle trituration every 5 minutes until

(18) Loss of diacylglycerol kinase ε causes thrombotic microangiopathy by impairing endothelial VEGFA signalingJCI insightMay 10, 2021Liu D, Ding Q, Dai DF, Padhy B, Nayak MK, Li C, Purvis M, Jin H, Shu C, Chauhan AK, et al.2 mL of protease solution — 5 mM CaCl2, 10 mg/mL Bacillus licheniformis protease (Creative Enzymes, NATE0633), and 125 U/mL DNase I (Roche) in Dulbecco’s PBS — on ice for 30 minutes. The lysates were filtered

(19) Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responsesGenome BiologyDecember 1, 2019Ciara H. O’Flanagan et al.protease, subtilisin A, derived from the Himalayan soil bacterium Bacillus lichenformis (Creative Enzymes NATE0633) in PBS supplemented with 5 mM CaCl2 and 125 U/ml DNAse, as described in [6, 31]. During dissociation,

(20) Single-Cell Profiling Reveals Sex, Lineage, and Regional Diversity in the Mouse KidneyDevelopmental cellNovember 4, 2019Ransick A, Lindström NO, Liu J, Zhu Q, Guo JJ, Alvarado GF, Kim AD, Black HG, Kim J, McMahon AP.collagenase, type 2 [Worthington, #LS00417]; 7.5 mg/ml B. licheniformis protease [Creative Enzymes, NATE-0633] and 125 U/ml DNase I [Worthington, #LS002058]. At five minute intervals, each digestion was mixed twenty

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Native Bacillus licheniformis Protease from Creative Enzymes

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Native Bacillus licheniformis Protease from Creative Enzymes

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