FGR (NM_005248) Human Recombinant Protein PH36140M5 from Leading Biology

Product Specs
ItemFGR (NM_005248) Human Recombinant Protein
CompanyLeading Biology
Price $552.00Get Pricing Get Quote for this Product
Catalog NumberPH36140M5
FormatLiquid
Conjugate/TagC-Myc/DDK
Concentration>50 ug/mL as determined by microplate BCA method
Size20 ug
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
NCBI Full Gene NameFGR proto-oncogene, Src family tyrosine kinase
NCBI Gene Aliasesp58-Fgr, p55-Fgr, SRC2, p55c-fgr, p58c-fgr, c-fgr, c-src2
Research AreaChemokine signaling pathway
Molecule NameFGR
TypeRecombinant Proteins
Source/Expression SystemHEK293T
Molecular Weight59.3 kDa
SpeciesHuman
ActivityFGR activity verified in a biochemical assay: FGR (Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. FGR is a tyrosine kinase that is a member of the Src family of protein tyrosine kinases. Varying concentrations of FGR were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors