ALK (NM_004304) Human Recombinant Protein PH34203M5 from Leading Biology

Product Specs
ItemALK (NM_004304) Human Recombinant Protein
CompanyLeading Biology
Price $552.00Get Pricing Get Quote for this Product
Catalog NumberPH34203M5
FormatLiquid
Conjugate/TagC-Myc/DDK
Concentration>50 ug/mL as determined by microplate BCA method
Size20 ug
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
NCBI Full Gene NameALK receptor tyrosine kinase
NCBI Gene AliasesCD246, NBLST3
Molecule NameALK
TypeRecombinant Proteins
Source/Expression SystemHEK293T
Molecular Weight176.3 kDa
SpeciesHuman
ActivityALK activity verified in a biochemical assay: ALK (anaplastic lymphoma receptor tyrosine kinase) (PH34203M5) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. ALK is an orphan receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. Varying concentrations of ALK were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors