CSK (NM_004383) Human Recombinant Protein PH37117M5 from Leading Biology

Product Specs
ItemCSK (NM_004383) Human Recombinant Protein
CompanyLeading Biology
Price $552.00Get Pricing Get Quote for this Product
Catalog NumberPH37117M5
Size20 ug
FormatLiquid
Conjugate/TagC-Myc/DDK
Concentration>50 ug/mL as determined by microplate BCA method
ActivityCSK activity verified in a biochemical assay: CSK (c-src tyrosine kinase) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. CSK is a tyrosine kinase known to phosphorylate LCK, FYN and LYN. Varying concentrations of CSK were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the tyrosine residue in the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
Molecule NameCSK
TypeRecombinant Proteins
Source/Expression SystemHEK293T
Molecular Weight50.5 kDa
SpeciesHuman
NCBI Full Gene NameC-terminal Src kinase
Research AreaChemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, Regulation of actin cytoskeleton