LAP3 (NM_015907) Human Recombinant Protein PH37823M5 from Leading Biology

Product Specs
ItemLAP3 (NM_015907) Human Recombinant Protein
CompanyLeading Biology
Price $552.00Get Pricing Get Quote for this Product
Catalog NumberPH37823M5
FormatLiquid
Conjugate/TagC-Myc/DDK
Concentration>50 ug/mL as determined by microplate BCA method
Size20 ug
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
NCBI Full Gene Nameleucine aminopeptidase 3
NCBI Gene AliasesPEPS, LAP, LAPEP, HEL-S-106
Research AreaArginine and proline metabolism, Glutathione metabolism, Metabolic pathways
Molecule NameLAP3
TypeRecombinant Proteins
Source/Expression SystemHEK293T
Molecular Weight56 kDa
SpeciesHuman
ActivityLAP3 activity verified in a biochemical assay: Leucine aminopeptidase 3 (LAP3) activity was measured in a fluorescent biochemical assay. LAP3 catalyzes the removal of unsubstituted N-terminal amino acids from various peptides and is most active on leucine. LAP3 activity was measured in a 100 µl reaction mixture containing 1 mM L-leucine 7-amido-4-methyl coumarin (Leu-AMC), 50 mM Tris, pH 8.0, 4 mM MgCl2, and 1 mM MnCl2. Cleavage of leucine from the AMC moiety results in a strong increase in fluorescence intensity. Fluorescence was measured over time with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. The activity of the enzyme in this system remained constant over six hours