Fig 1: Ccn1 expression in PDAC correlates with immunosuppression. A) Tumor growth curves of sgCtrl and sgCcn1 KPC cells subcutaneously inoculated in C57B/L6 mouse. n = 6 mice per group. B) Representative images of tumors (left) and quantification of tumor weight (right) in sgCtrl and sgCcn1 KPC cell‐inoculated mice. C) Representative images of H&E, and immunohistochemistry analysis for Ki67, CCN1, CD45, F4/80, CD4, CD8, and Gr, markers in sgCtrl and sgCcn1 KPC subcutaneous tumors. D) Flow cytometric quantification of CD8+ T cells, CD4+ T cells, macrophages, MDSCs, and IFNγ+ CD8+ T cells in sgCtrl and sgCcn1 KPC tumors. E) Protein levels of TNFα, IFNγ, IL2, IL6 in sgCtrl and sgCcn1 KPC cells inoculated subcutaneous tumors. F) Protein levels of TNFα, IFNγ, IL2, IL6 in serum from mice bearing sgCtrl and sgCcn1 tumors. G) mRNA levels of Tnfa, Il2, Il6, and Il8 in sgCtrl and sgCcn1 KPC tumors.