Fig 1: Fibroblastic mutant p53 upregulates SAA1, SAA2, and THBS4 in the NP mammary gland, promoting neu tumor cell proliferation and migration.a MA-plot comparing mammary glands from NP (MGNP) and N (MGN) mice. Red and blue dots indicate significantly upregulated and downregulated genes, respectively (adjusted p-value < 0.05); gray dots represent non-significant changes. b Box plots show eight upregulated and seven downregulated secretory genes with adjusted p-values < 0.05. Gene expression levels in MGNP are shown in red, and those in MGN are shown in cyan. c MTT assay results showing the effects of secretory peptides on HER2/neu+ mammary tumor cell proliferation. Cells were treated with either vehicle control, SAA1, SAA2, or THBS4 peptides, and cell viability was assessed at 1, 2, or 3 days post-treatment. All comparisons to the vehicle control yielded p < 0.05, except for the optical density measured 3 days after SAA2 treatment. d Representative images from Boyden chamber assays showing the migration of HER2/neu+ mammary tumor cells in response to secretory peptides, SAA1, SAA2, or THBS4. Ctrl, vehicle control. e Transcriptional expression of SAA1 and SAA2 in breast cancer samples from the TCGA and METABRIC databases, stratified by TP53 status (WT, wild-type vs. Mut, mutant).