Western blot analysis showing detection of 67 kDa 4-Hydroxynonenal BSA Conjugate (SPR-211) using Anti-4-Hydroxynonenal Antibody, Clone 12F7 (SMC-511). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (1.0 µg). Lane 4: 4-Hydroxynonenal BSA (SPR-211) (0.5 µg). Lane 5: 4-Hydroxynonenal BSA (1.0 µg). Block: 5% Skim Milk in 1X TBST. Primary Antibody: Anti-4-Hydroxynonenal Antibody, Clone 12F7 (SMC-511) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.