TEM of ATTO 594-conjugated alpha synuclein PFFs (SPR-322-A594)
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. ATTO 594-conjugated alpha synuclein pre-formed fibrils (SPR-322-A594) show similar seeding capabilities as uncojugated alpha synuclein pre-formed fibrils (SPR-322) when combined with monomers. Plate Used: HTRF 96-well low volume white plate (Cisbio). Reaction Volume: 15 uL. Thioflavin T Concentration: 40 uM. SDS Catalyst concentration: 0.73 mM. Incubation: 37C, 600 rpm.
Supplier Page from StressMarq Biosciences Inc for Alpha Synuclein Pre-formed Fibrils: ATTO 594