Western blot analysis showing detection of 67 kDa 4-Hydroxy-2-hexenal BSA Conjugate (SPR-210) using Anti-4-Hydroxy-2-hexenal Antibody, Clone 6F10 (SMC-510). Lane 1: Molecular Weight Ladder (MW). Lane 2: 4-hydroxy-2-hexanal-BSA (SPR-210) (1.0 µg). Lane 3: 4-hydroxy-2-hexanal-BSA (SPR-210) (0.5 µg). Lane 4: 4-hydroxy-2-hexanal-BSA (SPR-210) (0.1.0 µg). Block: 5% Skim Milk in 1X TBST. Primary Antibody: Anti-4-Hydroxy-2-hexenal Antibody, Clone 6F10 (SMC-510) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Supplier Page from StressMarq Biosciences Inc for 4-Hydroxy-2-hexenal BSA Conjugate