Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated 10377-H41H-B from Sino Biological, Inc.

Product Specs
ItemHuman PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated
CompanySino Biological, Inc.
Price $298.00Supplier Page View Company Product Page
Catalog Number10377-H41H-B
TypeRecombinant protein
Molecule NamePDCD1
Conjugate/TagC-human IgG1-Fc, AVI
SpeciesHuman
NCBI Full Gene Nameprogrammed cell death 1
NCBI Gene AliasesCD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1
FormatLyophilized from sterile PBS, pH 7.4
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant human PDCD1 consists of 396 amino acids and predicts a molecular mass of 44.49 kDa. As a result of glycosylation, it migrates as an approximately 61.93 KDa band in SDS-PAGE under reducing conditions
Purity≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC
Activity1.Immobilized Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat: 10377-H41H-B) at 2 µg/mL (100 µl/well) can bind Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B), the EC50 is 40-130 ng/mL (QC tested). 2.Immobilized Anti-PD1 (Research Grade Nivolu*mab Biosimilar) at 2 µg/mL (100 µL/well) can bind Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B), the EC50 is 0.6-2 ng/mL (Routinely tested). 3.Immobilized Recombinant Human B7-DC / PD-L2 / CD273 / PDCD1LG2 Protein (Fc Tag) (Cat: 10292-H02H) at 2 µg/mL (100 µL/well) can bind Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B), the EC50 is 3-10 ng/mL (Routinely tested). 4.Immobilized Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B) at 1 µg/mL (100 µl/well) on streptavidin precoated (2 µg/mL, 100 µL/well) can bind Recombinant Mouse B7-DC / PD-L2 / CD273 Protein (His Tag) (Cat: 50804-M08H), the EC50 is 13.3 ng/mL (Routinely tested). 5.Serial dilutions of Anti-PD1 (Research Grade Nivolu*mab Biosimilar) were added into Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B): Recombinant Human PD-L1 / B7-H1 / CD274 Protein (ECD, mFc Tag) (Cat: 10084-H05H) binding reactions. The half maximal inhibitory concentration (IC50) is 0.3 µg/mL (Routinely tested)
Size20 µg