Human GM-CSF Recombinant Protein PHC2013 from Thermo Fisher Scientific

Product Specs
ItemHuman GM-CSF Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHC2013
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size1 mg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) microRNA-132 attenuates inflammation in induced pluripotent stem cell-derived microglia from Alzheimer’s disease patientsActa Neuropathologica CommunicationsMarch 7, 2026Amber Penning, Sarah Snoeck, Olmo Ruiz Ormaechea, Dilara Ayyildiz, Oliver Polzer, Martin Buitrago-Arango, Raffaella Capobianco, Fred de Winter, Sriram Balusu, Joost Verhaagen, et al.IL-34 Recombinant Protein (Cat#200–34, Peprotech), and 10 ng/mL Human GM-CSF Recombinant Protein (Cat#PHC2013, Thermo Fisher Scientific). Medium changes of 50% occurred three times per week. iPSCs were differentiated

(2) Bacteria enable tolerance to bile salt exposure in an immune-competent human intestinal modelGut MicrobesDecember 31, 2025Yuan Li, Chia-Ming Wang, Peng Zhao, Matthew T. Fernez, Rebecca L. Carrierpenicillin-streptomycin, 10 nM retinoic acid (Sigma-Aldrich, 302–79–4), 100 ng/mL human GM-CSF recombinant protein (Gibco, PHC2015), 70 ng/mL recombinant human IL-4 Protein (SinoBiological 11,846-HNAE), 10% heat-inactivated fetal bovine

(3) RERE-AS1 as a regulator of immune modulation and therapeutic response in breast cancerCancer Immunol ImmunotherDecember 19, 2025Shaohui Jiang, Weijie He, Zhimin Li, Haodong He, Yuehua Li, Meiling Wen, Hongbo Zhu, Pengfei LuoLtd.). Monocytes were cultured in RPMI-1640 enriched with 10% FBS and 10 ng/mL recombinant human GM-CSF (PHC2013, Gibco, USA) for 7 days to induce differentiation into M1/M2 macrophages or dendritic cells (DCs). In

(4) IgA2 ACPA Drives a Hyper-Inflammatory Phenotype in Macrophages via ATP Synthase and COX2Eur J ImmunolApril 1, 2025Luís Almeida, Alice Bacon, Mohan Ghorasaini, Alwin J. van der Ham, René E. M. Toes, Martin Giera, Bart EvertsU/mL penicillin/streptomycin, and 2 mM L‐glutamine) supplemented with 20 ng/mL rGM‐CSF (ThermoFisher, PHC2011). On day 2/3, the medium, including supplements, was replaced. Macrophages were stimulated on day 5

(5) Constitutive activation of the Src-family kinases Fgr and Hck enhances the tumor burden of acute myeloid leukemia cells in immunocompromised miceSci RepJanuary 2, 2025Sherry T. Shu, Li Chen, Giancarlo Gonzalez-Areizaga, Thomas E. Smithgallcells were cultured in the same medium supplemented with 100 ng/mL human GM-CSF (Life Technologies #PHC2015). Human 293T cells were cultured in DMEM with 10% FBS. Expression vector construction and retroviral

(6) AMPK activation induces RALDH+ tolerogenic dendritic cells by rewiring glucose and lipid metabolismThe Journal of cell biologyOctober 7, 2024Brombacher EC, Patente TA, van der Ham AJ, Moll TJA, Otto F, Verheijen FWM, Zaal EA, de Ru AH, Tjokrodirijo RTN, Berkers CR, et al.Sigma-Aldrich), and 2 mM L-glutamine (G8540; Sigma-Aldrich) supplemented with 10 ng/ml human rGM-CSF (PHC2013; Gibco) and 0.86 ng/ml human rIL4 (204-IL; R&D Systems) to induce moDC differentiation. On day 2/3,

(7) Human-specific paralogs of SRGAP2 induce neotenic features of microglia structural and functional maturationbioRxivSeptember 16, 2024Carlos Diaz-Salazar, Marine Krzisch, Juyoun Yoo, Patricia R. Nano, Aparna Bhaduri, Rudolf Jaenisch, Franck PolleuxScientific,RHBMP-4 SCF,Life Technologies,PHC2115 VEGF-121,Life Technologies,PHC9394 M-CSF,Life Technologies,PHC2015 IL-3,Life Technologies,PHC0034 Protamine sulfate,Thermo Scientific,J62926.06 Zeocin,Thermo Scientific,R25001 Dulbecco’s

(8) Profiling migration of human monocytes in response to chemotactic and barotactic guidance cuesCell Reports MethodsSeptember 16, 2024Clare K. Hall, Olivia M. Barr, Antoine Delamare, Alex Burkholder, Alice Tsai, Yuyao Tian, Felix E. Ellett, Brent M. Li, Rudolph E. Tanzi, Mehdi Jorfipreviously described and resuspended at a concentration of 1,000,000 cells/mL in 10 ng/mL GM-CSF (Gibco, PHC2015) in IMDM +1% penicillin/streptomycin (Gibco, 10378016) for 15 h or 50 ng/mL M-CSF (Gibco, PHC9501) in

(9) Metabolic heterogeneity of tissue-resident macrophages in homeostasis and during helminth infectionNature CommunicationsSeptember 12, 2023Graham A. Heieis, Thiago A. Patente, Luís Almeida, Frank Vrieling, Tamar Tak, Georgia Perona-Wright, Rick M. Maizels, Rinke Stienstra, Bart Evertsof penicillin, 100 μg/mL streptomycin, 2 mM of glutamine, and either 20 ng/mL rGM-CSF (ThermoFisher, PHC2011) or 20 ng/ml rM-CSF (ThermoFisher, PHC9501) for 6 days. Cultures were supplemented on day 2/3 with an

(10) microRNA-132 regulates gene expression programs involved in microglial homeostasisiScienceJune 16, 2023Hannah Walgrave, Amber Penning, Giorgia Tosoni, Sarah Snoeck, Kristofer Davie, Emma Davis, Leen Wolfs, Annerieke Sierksma, Mayte Mars, Taofeng Bu, et al.β-mercaptoethanol, 50 U/ml penicillin/streptomycin, 100 ng/mL IL34 (Cat#200–34, Peprotech) and 10 ng/mL GM-CSF (Cat#PHC2013, Thermo Fisher Scientific). Microglia differentiation medium was changed approximately 3 times per week. Neuronal

(11) Thymoquinone Effect on Monocyte-Derived Macrophages, Cell-Surface Molecule Expression, and PhagocytosisNutrientsDecember 8, 2022Nuha A. Alkhattabi, Sowsan A. Hussein, Nesrin I. Tarbiah, Reem Y. Alzahri, Reham Khalifagranulocyte-macrophage colony-stimulating factor granulocyte-macrophage colony stimulating factor (GM-CSF) (PHC2015, ThermoFisher, Waltham, MA, USA) at a final concentration of 10 ng/mL. Cells were incubated for 7 days

(12) Novel potent liposome agonists of triggering receptor expressed on myeloid cells 2 phenocopy antibody treatment in cellsGliaDecember 1, 2022Boudesco C, Nonneman A, Cinti A, Picardi P, Redaelli L, Swijsen S, Roewe J, Reinhardt P, Ibach M, Walter J, et al.(ThermoFisher, #31350‐010), 100 ng/ml IL‐34 (PeproTech, #200–34) and 10 ng/ml GM‐CSF (ThermoFisher, #PHC2015) in uncoated μClear 96‐well plates (Greiner Bio‐one, #655090) at a density of 30,000 cells/well in a

(13) A Costimulatory CAR Improves TCR-based Cancer ImmunotherapyCancer immunology researchApril 1, 2022Omer B, Cardenas MG, Pfeiffer T, Daum R, Huynh M, Sharma S, Nouraee N, Xie C, Tat C, Perconti S, et al.cultured in DC media (CellGenix, 20801-0500), supplemented with 800 U/mL GMCSF (Thermo Fisher Scientific, PHC2011) and 1,000 U/mL of IL4 (R&D Systems, 204-IL) for 5 days, with GMCSF and IL4 replenishment on day 3.

(14) Epstein-Barr Virus-Encoded Circular RNA CircBART2.2 Promotes Immune Escape of Nasopharyngeal Carcinoma by Regulating PD-L1Cancer researchOctober 1, 2021Ge J, Wang J, Xiong F, Jiang X, Zhu K, Wang Y, Mo Y, Gong Z, Zhang S, He Y, et al.(45-001-749, Cytiva) separation solution and added to 10% FBS-1640 medium containing 50 ng/m LGM-CSF (PHC2013, Invitrogen) and 20 ng/mL IL4 (PHC0044, Invitrogen) for 5 days. To promote the maturation of dendritic cells

(15) Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteinsCell reportsMay 11, 2021Hiatt J, Cavero DA, McGregor MJ, Zheng W, Budzik JM, Roth TL, Haas KM, Wu D, Rathore U, Meyer-Franke A, et al.100μg/mL, respectively, Corning 30–002-CI), 1mM Sodium Pyruvate, 50ng/mL GM-CSF (Life Technologies PHC2015), 50ng/mL IL-4 (Life Technologies PHC0045); for macrophage differentiation: 20% Human AB Serum, Penicillin-Streptomycin,

(16) Biological and molecular studies on specific immune cells treated with checkpoint inhibitors for the thera-personal approach of breast cancer patients (ex-vivo study)Oncology researchJanuary 1, 2021El-Houseini ME, Arafat MS, El-Husseiny AM, Kasem IM, Kamel MM, El-Habashy AH, Khafagy MM, Radwan EM, Helal MH, Abdellateif MS.human granulocyte monocyte-colony-stimulating factor (GM-CSF; 50 ng/ml, Gibco-Life Technology, Cat. no. PHC2013), interleukin 4 (IL4; 10 ng/ml, Bio Basic Inc-Cat. no. RC212-15), IL12 (20 ng/ml, Bio Basic Inc-Cat.

(17) HIV-1 Vpr induces cell cycle arrest and enhances viral gene expression by depleting CCDC137eLifeJune 15, 2020Zhang F, Bieniasz PD.paper,,tetracycline-inducible CCDC137 expression pLKO vector "Peptide, recombinant protein",GM-CSF,Thermo Fisher,Cat# PHC2011,Macrophage differentiation Commercial assay or kit,FxCycle PI/RNase Staining Solution,Invitrogen,Cat#:

(18) Epigenetic Suppression of HIV in Myeloid Cells by the BRD4-Selective Small Molecule Modulator ZL0580Journal of virologyMay 18, 2020Alamer E, Zhong C, Liu Z, Niu Q, Long F, Guo L, Gelman BB, Soong L, Zhou J, Hu H.0.3 mg/ml), 10% (vol/vol) FBS, and 100 ng/ml granulocyte-macrophage colonystimulating factor (Gibco, PHC2015) for 6 days. At day 3 of cell culture, half of the culture medium was replaced with fresh differentiation

(19) The loss of microglia activities facilitates glaucoma progression in association with CYP1B1 gene mutation (p.Gly61Glu)PloS oneJanuary 1, 2020Alghamdi A, Aldossary W, Albahkali S, Alotaibi B, Alrfaei BM.Finally, the medium containing the microglia was placed into new flasks along with GM-CSF (Catalogue #PHC2013, Thermo Fisher Scientific, USA) growth factors. Additionally, new media with 10% FBS were added to the

(20) Generation and comprehensive characterization of induced pluripotent stem cells for translational researchRegenerative medicineJune 1, 2019MacArthur CC, Pradhan S, Wetton N, Zarrabi A, Dargitz C, Sridharan M, Jackson S, Pickle L, Lakshmipathy U.OpTmizer SFM containing 200 mM GlutaMax, 100 ng/ml SCF, 50 ng/ml IL-3 and 25 ng/ml GM-CSF (catalog # PHC2015). iPSC generation Somatic cells were reprogrammed using Sendai virus based CytoTune iPS 2.0 kit (CytoTune

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Human GM-CSF Recombinant Protein from Thermo Fisher Scientific

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Human GM-CSF Recombinant Protein from Thermo Fisher Scientific

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