Human HGF Recombinant Protein PHG0321 from Thermo Fisher Scientific

Product Specs
ItemHuman HGF Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHG0321
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size100 µg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Effect of triazine thiols on steady-state mRNA levels in iPSC-derived hepatocytesMicroPubl BiolMarch 6, 2026Carla Martinez-Morant, Jui-Tung Liu, Yu-Lin Jiang, Josef Blaszkiewicz, Stephen A. Duncanreceived an additional 5 days of B27 Supplement and 20 ng/mL Hepatocyte Growth Factor (Invitrogen, MA, #PHG0321) to generate immature hepatocytes. Finally, the cells were cultured in HCM medium (Lonza, MD, #CC3198)

(2) Covalent inhibitor design confers activity against both GDP- and GTP-bound forms of KRAS G12CNature CommunicationsJanuary 31, 2026Matthew L. Condakes, Zhuo Zhang, Derek B. Danahy, Richard R. Moore, Sirish Kaushik Lakkaraju, Xiaoliang Zhuo, Yuka Amako, Robert M. Borzilleri, Srividya B. Balachander, Lisa Chourb, et al.human epidermal growth factor (ThermoFisher: PHG0315) or human hepatocyte growth factor (ThermoFisher: PHG0324) was added to the culture medium. Cells were treated for 5 days, and the viability signal was obtained

(3) Transient proliferation by reversible YAP and mitogen control of the cyclin D1/p27 ratioCommunications BiologyJanuary 29, 2026Katherine R. Ferrick, Samsara W. Upadhya, Yilin Fan, Nalin Ratnayeke, Mary N. Teruel, Tobias Meyer25 ng/mL recombinant EGF (PeproTech cat#AF-100-15), 25 ng/mL recombinant HGF (Life Technologies Cat#PHG0254), 25 ng/mL recombinant FGF basic (R&D Systems Cat#233-FB-025), 1 µM LPA (oleoyl-L-alpha-lysophosphatidic

(4) Monocyte-derived macrophages support alveolar regeneration via oncostatin M post-H1N1 infection during the recovery phaseRespir ResOctober 8, 2025Xiao Shang, Ju Jia, Jiapei Yu, Shumei Zou, Zeyi Wang, Jiuyang Xu, Hui Li, Bin Caofactor (#PMG8044, Gibco), keratinocyte growth factor (#PHG0094, Gibco), hepatocyte growth factor (#PHG0324, Gibco), and fibroblast growth factor-basic (#PMG0034, Gibco) in DMEM-F12. Rock inhibitor (#C9903, Sigma)

(5) Blood-brain barrier permeability increases with the differentiation of glioblastoma cells in vitroFluids and barriers of the CNSNovember 1, 2024Digiovanni S, Lorenzati M, Bianciotto OT, Godel M, Fontana S, Akman M, Costamagna C, Couraud PO, Buffo A, Kopecka J, et al.supplement, 2% B27 (#17504044), 10 ng/mL PDGFa (#PHG0035), 10 ng/mL IGF-1 (#PHG0078), 5 ng/mL HGF (#PHG0254), 10 ng/mL NT3 (#PHC7036; all from Thermofisher Scientific), and 1% rh-insulin (#I3536), 60 ng/mL T3

(6) Proteo-metabolomics and patient tumor slice experiments point to amino acid centrality for rewired mitochondria in fibrolamellar carcinomaCell Reports MedicineSeptember 17, 2024Donald Long, Marina Chan, Mingqi Han, Zeal Kamdar, Rosanna K. Ma, Pei-Yin Tsai, Adam B. Francisco, Joeva Barrow, David B. Shackelford, Mark Yarchoan, et al.for 30 min, 1% penicillin-streptomycin (Gibco 15140122), and 2.5 ng/mL hepatic growth factor (Gibco PHG0321). Method details Nutrient manipulation assays Cell media (stock) was generated for the FLC cell

(7) Differential Immune Checkpoint Protein Expression in HNSCC: The Role of HGF/MET SignalingInternational Journal of Molecular SciencesJuly 4, 2024Verena Boschert, Johannes Boenke, Ann-Kathrin Böhm, Jonas Teusch, Valentin Steinacker, Anton Straub, Stefan Hartmannsamples were prepared as described elsewhere [40]. HGF was obtained from Thermo Fisher Scientific (PHG0254, Waltham, MA, USA) or Abcam (Ab259401, Cambridge, Great Britain). Foretinib was purchased from Selleck

(8) A PNPLA3-Deficient iPSC-Derived Hepatocyte Screen Identifies Pathways to Potentially Reduce Steatosis in Metabolic Dysfunction-Associated Fatty Liver DiseaseInternational Journal of Molecular SciencesJuly 2, 2024Caren Doueiry, Christiana S. Kappler, Carla Martinez-Morant, Stephen A. DuncanRPMI supplemented with B27 Supplement and 20 ng/mL of Hepatocyte Growth Factor (Invitrogen, MA, USA, #PHG0321) to generate immature hepatocytes. Finally, the cells were cultured in HCM medium (Lonza, Walkersville,

(9) A map of signaling responses in the human airway epitheliumCell SystemsApril 17, 2024Katherine B. McCauley, Kalki Kukreja, Alfredo E Tovar Tovar Walker, Aron B. Jaffe, Allon M. KleinFGF2,ThermoFisher,PHC9534 CHIR99021,Stem Cell Technologies,72052 Recombinant human HGF,ThermoFisher,PHG0254 Human IFN-Alpha (alpha 2A),PBL Assay Science,11100 Recombinant human IFN-Gamma,Peprotech,300–02 Recombinant human IL-13

(10) Xeno-Free 3D Bioprinted Liver Model for Hepatotoxicity AssessmentInternational Journal of Molecular SciencesFebruary 2, 2024Ahmed S. M. Ali, Johanna Berg, Viola Roehrs, Dongwei Wu, Johannes Hackethal, Albert Braeuning, Lisa Woelken, Cornelia Rauh, Jens KurreckGibco, Life Technologies Corp, Grand Island, NY, USA), recombinant human hepatocyte growth factor (HGF, PHG0254, Gibco, Life Technologies Corp, Frederick, MD, USA), or recombinant human epidermal growth factor (EGF,

(11) Improvements in Maturity and Stability of 3D iPSC-Derived Hepatocyte-like Cell CulturesCellsSeptember 27, 2023Suominen S, Hyypijev T, Venäläinen M, Yrjänäinen A, Vuorenpää H, Lehti-Polojärvi M, Räsänen M, Seppänen A, Hyttinen J, Miettinen S, et al.were cultured in HBM (cc-3199, Lonza) supplemented with single quotes complemented with 25 ng/mL HGF (PHG0254, Life Technologies) and 20 ng/mL oncostatin M (OSM, 295-OM, R&D Systems). 2.3. Isolation and Culture

(12) Deciphering the Role of p60AmotL2 in Epithelial Extrusion and Cell DetachmentCellsAugust 28, 2023Cui W, Subramani A, Fonseca P, Zhang Y, Tong L, Zhang Y, Egevad L, Lundqvist A, Holmgren L.p60AmotL2 expression. Subsequently, they were treated with Hepatocyte Growth Factor (HGF, product number PHG0254, Thermo Fisher Scientific, Waltham, MA, USA) for the following 3 days. The cells, along with the collagen

(13) Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenomaScientific reportsJuly 6, 2023Yang KS, O'Shea A, Zelga P, Liss AS, Del Castillo CF, Weissleder R.ng/mL epidermal growth factor (Sigma-Aldrich, E9644), and 10 ng/mL hepatocyte growth factor (Thermo, PHG0324). Other cell lines used as positive or negative controls were grown according to ATCC in media with

(14) Dual-targeting therapy against HER3/MET in human colorectal cancersCancer medicineApril 1, 2023Yamasaki A, Miyake R, Hara Y, Okuno H, Imaida T, Okita K, Okazaki S, Akiyama Y, Hirotani K, Endo Y, et al.Anti‐HER3 mouse mAb (#sc‐7390, Santa Cruz, CA, USA) was also used. NRG1 (CYT‐407, ProSpec), and HGF (PHG0254, Invitrogen) were added to the cell culture with FBS‐free RD medium. The tyrosine kinase inhibitor for

(15) Coronary artery disease patient-derived iPSC-hepatocytes have distinct miRNA profile that may alter lipid metabolismScientific reportsJanuary 30, 2023Alexanova A, Raitoharju E, Valtonen J, Aalto-Setälä K, Viiri LE.medium prepared with HCM Hepatocyte BulletKit (Lonza, CC-3198) and supplemented with 25 ng/ml HGF (PHG0254, Thermo Scientific) and 20 ng/ml Oncostatin M (OSM, 295-OM, R&D systems). Primary human hepatocytes

(16) MET Exon 14 Splice-Site Mutations Preferentially Activate KRAS Signaling to Drive TumourigenesisCancersMarch 8, 2022Lu D, Nagelberg A, Chow JL, Chen YT, Michalchuk Q, Somwar R, Lockwood WW.Fisher Scientific, cat #BP231-100) prior to use. Hepatocyte Growth Factor recombinant human protein (PHG0324) was purchased from Thermo Scientific and reconstituted according to manufacturer’s instruction prior

(17) PKM2 Modulation in Head and Neck Squamous Cell CarcinomaInternational Journal of Molecular SciencesJanuary 11, 2022Verena Boschert, Jonas Teusch, Urs D. A. Müller-Richter, Roman C. Brands, Stefan Hartmannfor 48 h and prepared for Western blot analysis as described elsewhere [37]. FGF2 (PHG0266) and HGF (PHG0254) were obtained from Thermo Fisher Scientific (Waltham, MA, USA), and EGF (E9644) from Merck (Darmstadt,

(18) Two-Chains Tissue Plasminogen Activator Unifies Met and NMDA Receptor Signalling to Control Neuronal SurvivalInternational journal of molecular sciencesDecember 15, 2021Hedou E, Douceau S, Chevilley A, Varangot A, Thiebaut AM, Triniac H, Bardou I, Ali C, Maillasson M, Crepaldi T, et al.either sc-tPA (300 nM), tc-tPA (300 nM), recombinant HGF (50 ng/mL; rHGF; Gibco, Life Technologies PHG0254, Saint Aubin, France), anti-HGF blocking (50 ng/mL; Santa-Cruz Biotechnology sc-1356), DO-24 antibody

(19) Activation of Receptor Tyrosine Kinases Mediates Acquired Resistance to MEK Inhibition in Malignant Peripheral Nerve Sheath TumorsCancer ResearchFebruary 1, 2021Jiawan Wang, Kai Pollard, Ana Calizo, Christine A. Pratilasby Dr. Vijaya Ramesh at Massachusetts General Hospital (Boston, MA). Recombinant human HGF (rhHGF, #PHG0324) and PDGF (#PHG0045) were purchased from Thermo Fisher Scientific. Doxycycline was from Sigma Aldrich;

(20) GDNF drives rapid tubule morphogenesis in a novel 3D in vitro model for ADPKDJournal of cell scienceJuly 16, 2020Dixon EE, Maxim DS, Halperin Kuhns VL, Lane-Harris AC, Outeda P, Ewald AJ, Watnick TJ, Welling PA, Woodward OM.and a growth factor cocktail of 40 ng/ml recombinant human hepatocyte growth factor (HGF; Invitrogen, PHG0324), 20 ng/ml recombinant human epidermal growth factor (EGF; Sigma, E9644), and 8.8 ng/ml recombinant

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Human HGF Recombinant Protein from Thermo Fisher Scientific

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Human HGF Recombinant Protein from Thermo Fisher Scientific

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