TRYPSIN/EDTA 100ML 100 ML R001100 from Thermo Fisher Scientific

Product Specs
ItemTRYPSIN/EDTA 100ML 100 ML
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberR001100
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size100mL
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Upregulation of LTF promotes left-sided colorectal cancer development via activating PI3K/AKT pathwayCell Adhesion & MigrationDecember 31, 2026Peng Chen, Mingrui Zhang, Xuefeng BaiPreparation (FASP) procedure, proteins were enzymatically cleaved into peptides with trypsin (catalog no. R001100, Gibco, China) [19]. After desalting on C18 cartridges, we dried the peptides via vacuum centrifugation.

(2) The AGO2 adaptor LIMD1 expands the functional and evolutionary reach of microRNA targetingSci AdvJuly 24, 2026Alex F. F. Crozier, Kunal M. Shah, Paul Grevitt, Anisha Thind, Eleni Maniati, Jun Wang, Kylie Shen, Diana Cox, Vinothini Rajeeve, Pedro Cutillas, et al.phosphate-buffered saline (PBS), detached with Trypsin/ethylenediaminetetraacetic acid (EDTA; Gibco, R001100) for ∼3 min, neutralized with DMEM (Sigma-Aldrich, D8437) containing 10% fetal bovine serum (Gibco,

(3) Panax notoginseng extract alleviates atopic dermatitis-like skin inflammation associated with suppression of the cGAS-STING pathwayFront PharmacolJuly 9, 2026Xu Zhu, Yichun Wang, Zhongyi Chen, Ziyan Zhao, Chunhao Yang, Peng Xu, Liping Qu, Feifei Wang, Fengkun XiaoCN: SCSP-5091). Dulbecco’s Modified Eagle Medium (DMEM) (CN: 10566016), Trypsin solution (0.25%) (CN: R001100), and Fetal Bovine Serum (FBS) (CN: 16000069) were obtained by Gibco (Thermo Fisher Scientific, Waltham,

(4) Targeted Sirtuin 3 Activation by Biomimetic Black Phosphorus Nanosheets Mitigates Sepsis-Induced Acute Kidney Injury through Yeast Mitochondrial Escape 1-Like 1 DeacetylationBiomaterials ResearchJune 29, 2026Yiqiong Yang, Rui Zuo, Yi Wang, Rumeng Liu, Yi Zhou, Jun Wang(Beyotime, China), Dulbecco’s Modified Eagle Medium (DMEM)/F12 Medium (11320033) (Gibco, USA), Protease (R001100) (Gibco, China), Cell Freeze-Liquid (C0210B-50 ml) and phosphate-buffered saline (PBS) Buffer (C0221A)

(5) PRMT6 inhibition or deficiency attenuates diabetic neuropathic pain in male mice and is associated with reduced spinal neuroinflammation, microgliosis, and altered p53-p21 signalingJournal of NeuroinflammationMay 29, 2026Yan Chu, Honghao Song, Mengqiu Deng, Yuanyuan Fang, Ruifeng Ding, Kesheng Huang, Xiaoyi Fan, Lei Peng, Yutong Yang, Huawei Wei, et al.removed, and tissues enzymatically digested (0.25% trypsin-EDTA, 100 U/mL DNase I, 15 min, 37 °C, #R001100 and #89836, Thermo Scientific). After quenching with DMEM/F12(11330057, Gibco) containing 10% FBS, cells

(6) IL-18 suppresses retinoblastoma growth while concomitantly inducing an inflammation-/stress-associated and immune-remodeling responseCancer Cell InternationalApril 20, 2026Zide Chen, Wanqian Luo, Dongyang Wei, Qi Wang, Qing Gou, Jiawei Cao, Qicong Mai, Xiumei Chen, Xiaowen Cai, Xiaoming Chen, et al.Streptomycin solution (100X) (C0222, Beyotime, China); RPMI 1640 medium (11875093, Gibco, China); Trypsin (R001100, Gibco, China); Animal Free IL-18 Protein, Mouse (His) (HY-P70642AF, Medchem Express, USA), Animal Free

(7) Electroacupuncture combined with rTMS promotes neuronal regeneration via DNMT1-mediated PI3K-AKT pathway in cerebral palsy modelEur J HistochemApril 20, 2026Juan Fan, Yu Ma, Liying Sun, Suhua Miao, Jianghong Hepreviously described. Briefly, dissected cortices were digested at 37°C for 15 min in 0.25% trypsin (R001100; Gibco, Rockville, MD, USA) containing 0.02% EDTA. Digestion was terminated by adding Dulbecco’s Modified

(8) Standardized Workflow for the Generation of Patient-Derived Glioblastoma SpheroidsMethods ProtocApril 3, 2026Giuseppa D'Amico, Alessandra Maria Vitale, Martina Di Marco, Alessandro Lo Giudice, Francesca Chiara Cecala, Francesco Cappello, Celeste Caruso BavisottoDE17-602E);Antibiotic-antimycotic (100X) (Gibco, Cat. no.: 15240062);Trypsin/EDTA Solution 100X (Gibco, Cat. no.: R001100);Type II collagenase 250 U/mL (Gibco, Cat. no.: 17101015);MEM Non-Essential Amino Acids Solution (100X)

(9) Propofol regulates METTL3-mediated PARP-1 m6A modification to promote Parthanatos to improve NSCLC chemotherapy resistanceScientific ReportsMarch 5, 2026Quan Ling, Kepeng Liu, Junlin Wen, Jin Liu, Yong ChenRiver Laboratory Animal Technology Co., Ltd., China). DMEM/F12 (11320033) (Gibco, China). Trypsin (R001100) (Gibco, China). Cell Freezing Solution (C0210B-50 ml), PBS Buffer (C0221A) (Beyotime, China). Primers

(10) KIF23 Overexpression Promotes Cell Viability, Migration, and Invasion via the Wnt/β-Catenin Signaling Pathway in Anaplastic Thyroid CarcinomaInt J EndocrinolFebruary 10, 2026Yongkang Wu, Changwei Zheng, Weijie Chen, Tuo XuCO2. When the cells reached 80%–90% confluency, they were passaged using 0.25% trypsin‐EDTA solution (R001100, Gibco, China). The present study only performed experiments in commercially purchased cell line, and

(11) Antitumor Activity of the ACC Inhibitor Firsocostat in Breast Cancer Cell Lines: A Proof-of-Concept In Vitro StudyPharmaceuticals (Basel)January 24, 2026Simona Picerno, Eugenia Giglio, Martina Giuseffi, Marcello Radino, Marzia Sichetti, Marisabel Mecca#11875093), Dimethyl sulfoxide (DMSO) (≥99.7% Sigma-Aldrich, #67-68-5), Trypsin–EDTA Solution (Gibco, #R001100), Trypan Blue Stain (Gibco, #15250-061), Dulbecco’s Phosphate-Buffered Saline (PBS) (Gibco, #14190144),

(12) Lactucopicrin promotes the autophagic degradation of MAP2K4/MKK4 by mediating CCDC50 palmitoylation to alleviate osteoarthritis progressionAutophagyJanuary 22, 2026Wenjun Li, Qijie Sun, Konghe Hu, Dongmei Tang, Cheng Yang, Yingchao Xie, Xiaodong Peng, Yongtao Deng, Jiansen Lu, Yong Qi, et al.Biotechnology,R0016 Fetal bovine serum,Sigma-Aldrich,12103C Amphotericin B,Gibco,"15,290,026" Trypsinization,Gibco,R001100 Cell Counting Kit-8,Beyotime Biotechnology,C0037 Penicillin/streptomycin,Gibco,"15,140,122" Lipofectamine

(13) HRS Degradation‐Induced Nicotinamide Deficiency in Placental Extracellular Vesicles Triggers Preeclampsia by Disrupting Maternal‐Fetal Immune HomeostasisAdvanced ScienceJanuary 14, 2026Haiyi Fei, Yuhan Lin, Xiu Liu, Xiaohong Zhu, Xiaowen Lu, Zhan Shi, Fan Jia, Shiqian Xu, Ling Fang, Lingling Jiang, et al.of NP‐EV Proteins and RNA by Proteases and RNases NP‐EVs were treated with Trypsin/EDTA Solution (R001100, Thermo Fisher, USA) or RNase A (R1253, Thermo Fisher, USA) at 37°C for 5 min to degrade protein and

(14) Darunavir analog precursors target mitochondrial metabolism in multiple myeloma and CLL.Cancer Cell InternationalDecember 23, 2025Marisabel Mecca, Marzia Sichetti, Enrico La Spina, Martina Giuseffi, Eugenia Giglio, Simona Picerno, Luciana Valvano, Teodora Statuto, Alessandro Santarsiere, Maria Funicello, et al.Fisher Scientific (GIBCO),"Cat#: 11,875,093" Trypsin–EDTA Solution,Thermo Fisher Scientific,Cat#: R001100 Dulbecco’s Phosphate Buffered Saline,Thermo Fisher Scientific,"Cat#: 14,190,144" L–Glutamine,Thermo Fisher Scientific

(15) Compound Tinglizi Decoction-Containing Serum Is Associated with Inhibition of PANoptosis in Cardiomyocytes via SIRT3-Related Chaperone-Mediated Autophagy of AIM2Int J Gen MedNovember 14, 2025Ren Huang, Junyu Tang, Wenjuan Huang, Yan Shang, Wenli Liu, Huajun Long, Rongwen Wan(100X) (C0222) (Beyotime, Shanghai, China). DMEM/F12 (11320033) (Gibco, Shanghai, China). Trypsin (R001100) (Gibco, Shanghai, China). Cell Freezing Solution (C0210B-50mL), PBS Buffer (C0221A) (Beyotime, Shanghai,

(16) Compound Tinglizi Decoction-Containing Serum Is Associated with Inhibition of PANoptosis in Cardiomyocytes via SIRT3-Related Chaperone-Mediated Autophagy of AIM2International Journal of General MedicineNovember 14, 2025Ren Huang, Junyu Tang, Wenjuan Huang, Yan Shang, Wenli Liu, Huajun Long, Rongwen Wan(100X) (C0222) (Beyotime, Shanghai, China). DMEM/F12 (11320033) (Gibco, Shanghai, China). Trypsin (R001100) (Gibco, Shanghai, China). Cell Freezing Solution (C0210B-50mL), PBS Buffer (C0221A) (Beyotime, Shanghai,

(17) Hypoxia drives cervical cancer progression via OCT4/ORAI3-dependent glycolysis and Ca2+ signalingCellular and Molecular Life SciencesNovember 6, 2025Lingjia Lu, Fenfen Wang, Ting Wang, Ziyu Xing, Peiyue Jiangto 80% confluence (4 × 105 cells per dish), the cells were trypsinized with 0.025% trypsin (Gibco, R001100). All cell lines used are listed using the official cell line name and its Research Resource Identifier

(18) Cachexia in mice blunts improved cognitive flexibility induced by calorie restrictionBrain, behavior, and immunityNovember 1, 2025Conn K, Milton LK, Teoh A, Levi PT, Walton KL, Andrews ZB, Foldi CJ, Lockie SH.cells, media was removed, cells were washed with PBS, then incubated in 3x trypsin enzyme (Gibco, #R001100) for 5 min at 37 °C. Cells were then collected with 10 ml of media to neutralise trypsin, then centrifuged

(19) Guilu Erxian oral liquid mitigates oxidative damage in spermatogonial cells via miR-6739-5p modulation and PI3K/AKT pathway activation: a functional histocytochemical studyEuropean Journal of HistochemistryOctober 2, 2025Zefeng Sun, Xinrong Fan, Zhenquan Liuspermatogonial stem cell complete culture medium (CM-R171, sourced from Procell in Wuhan, China), trypsin (R001100, Gibco, Rockville, MD, USA), cell freezing solution (C0210B-50 mL, Beyotime, Shanghai, China), PBS buffer

(20) Urolithin Α modulates inter-organellar communication via calcium-dependent mitophagy to promote healthy ageingAutophagySeptember 29, 2025Antonis Roussos, Katerina Kitopoulou, Fivos Borbolis, Christina Ploumi, Despoina D. Gianniou, Zhiquan Li, Haijun He, Eleni Tsakiri, Helena Borland, Ioannis K. Kostakis, et al.procedures applied, cells were subcultured by using a trypsin-EDTA solution (Thermo Fisher Scientific, R001100). HMC3 cells (ATCC, CRL-3304) were cultured in Minimum Essential Medium Eagle (Merck, M2279) containing

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TRYPSIN/EDTA 100ML 100 ML from Thermo Fisher Scientific

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TRYPSIN/EDTA 100ML 100 ML from Thermo Fisher Scientific

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