Human IL-3 Recombinant Protein PHC0035 from Thermo Fisher Scientific

Product Specs
ItemHuman IL-3 Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHC0035
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size50 µg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Tracking tau and cellular responses in human iPSC-microglia: from uptake to seedable secretion, including in extracellular vesiclesAlzheimers DementApril 6, 2026Maria Kreger Karabova, Anna Del Ser-Badia, Anne Hedegaard, Sam J. Washer, Zeynep Baykam, Darragh P. O'Brien, Iolanda Vendrell, Svenja S. Hester, Roman Fischer, Errin Johnson, et al.days, EBs were transferred to T175 flasks (“factories”) and cultured with 25 ng/mL IL‐3 (Catalog No. PHC0033, Invitrogen) and 100 ng/mL Macrophage Colony‐Stimulating Factor (M‐CSF)_ (Catalog No. PHC9501, Invitrogen)

(2) Targeting p75NTR activity alleviates the neurotoxic effect of high glucose on iPSC-derived dopaminergic neuronsStem Cell Research & TherapyMarch 21, 2026Konstantina Chanoumidou, Ioanna Zota, Maria Anna Papadopoulou, Chrystalla Konstantinou, Alexandros Tsimpolis, Electra Tsagliotis, Maria Tziortziou, Katerina Ntarntani, Anne Grünewald, Matthieu David Lavigne, et al.LZBE04-4418 F) medium supplemented with 100 ng/mL M-CSF (Invitrogen, PHC9501), 25 ng/mL IL-3 (Invitrogen, PHC0033), 2 mM Glutamax (Gibco, 35050), 100 U/mL penicillin and 100 µg/mL streptomycin (Gibco, 15140122), and

(3) microRNA-132 attenuates inflammation in induced pluripotent stem cell-derived microglia from Alzheimer’s disease patientsActa Neuropathologica CommunicationsMarch 7, 2026Amber Penning, Sarah Snoeck, Olmo Ruiz Ormaechea, Dilara Ayyildiz, Oliver Polzer, Martin Buitrago-Arango, Raffaella Capobianco, Fred de Winter, Sriram Balusu, Joost Verhaagen, et al.penicillin/streptomycin (Cat#P4333-100 mL, Sigma-Aldrich), 25 ng/mL Human IL-3 Recombinant Protein (Cat#PHC0035, Thermo Fisher Scientific), and 100 ng/mL Human M-CSF Recombinant Protein (Cat#PHC9501, Thermo Fisher Scientific

(4) Assessing Aβ-independent effects of Module 42 on immune function in vitroAlzheimers DementFebruary 25, 2026Ishita Ajith, Souvika Bakshi, Emma Mead, Opher Gileadi, Vittorio L. Katis, Paul E. Brennan, Emory‐Sage‐SGC‐Jax TREAT‐AD Center, Katerina O. Gospodinovacolony‐stimulating factor (M‐CSF; 100 ng/mL, PHC9501, Life Technologies), Interleukin‐3 (IL‐3; 25 ng/mL, PHC0031, Life Technologies), 2‐mercaptoethanol (50 µM, 31350010, Gibco), and penicillin/streptomycin (100 U/mL

(5) KCNA2 variants cause dilated cardiomyopathy, obesity and sleep apnea through RAC-ERK pathwayEMBO Molecular MedicineFebruary 24, 2026Prasanth Chimata, Sahil Lall, Tarmo Annilo, M K Mathew, Andres Metspalu, Jayaprakash Shenthar, Perundurai S DhandapanyFLT-3 at 100 ng/mL (Thermo Fisher Scientific, PHC9414), IL-3 at 20 ng/mL (Thermo Fisher Scientific, PHC0034), and IL-6 at 20 ng/mL (Thermo Fisher Scientific, PHC0065) using an ultra-low attachment 24-well plate

(6) The MYO1F interactome reveals ASAP1, CD2AP and SH3KBP1 as novel adaptor proteins in podosomes and phagosomesJournal of Cell ScienceNovember 10, 2025Susan D. Arden, Eva Pennink, András Lakatos, Gillian M. Griffiths, Anna H. Lippert, Folma Busswith 100 ng/ml M-CSF (Thermo Fisher Scientific, PHC9504), 25 ng/ml IL-3 (Thermo Fisher Scientific, PHC0035), 2 mM GlutaMAX, 0.05 mM β-mercaptoethanol (Thermo Fisher Scientific, 31350010) and 1× Antibiotic-Antimycotic

(7) Tubulin tyrosine ligase variant perturbs microtubule tyrosination, causing hypertrophy in patient-specific and CRISPR gene-edited iPSC-cardiomyocytesJCI InsightAugust 8, 2025Pratul Kumar Jain, Susobhan Mahanty, Harshil Chittora, Veronique Henriot, Carsten Janke, Minhajuddin Sirajuddin, Perundurai S. Dhandapany100 ng/mL [Invitrogen, PHC2115], FLT-3 100 ng/mL [Invitrogen, PHC9414], IL-3 20 ng/mL [Invitrogen, PHC0034], and IL-6 20 ng/mL [Invitrogen, PHC0064] at their final concentration). On day 0, cells were transduced

(8) Development of a robust BH3 drug toolkit for precision medicine in hematologic malignanciesTheranosticsApril 21, 2025Valentin Jacquier, Andréa Romero, Caroline Molinaro, Ritu Somayaji, Matthieu Abouladze, Ouissem Karmous Gadacha, Sara Ovejero, Hugues de Boussac, Ludovic Gabellier, Matthew S. Davids, et al.human FLT-3 Ligand (50 ng/mL, PHC9414, Gibco), human SCF (50 ng/mL, PHC2116, Gibco), IL-3 (10 ng/mL, PHC0034, Gibco) and IL-6 (10 ng/mL, 200-06, Preprotech, MA, USA). For diffuse large B-cell lymphoma (DLBCL)

(9) Role of IL3RA in a Family with Lumbar Spinal StenosisInternational journal of molecular sciencesOctober 10, 2024Liu KM, Yang CF, H'ng WS, Chuang HP, Khor EHX, Tsai PC, Khosasih V, Lu LS, Yeh EC, Lin WJ, et al.the medium was replaced with fresh medium, and the cells were incubated with 10 nM of IL-3 (Cat. No. PHC0031, GIBCO), 10 nM of β-estradiol (Cat. No. E8875, Sigma-Aldrich), and 15 or 30 nM of testosterone (Cat.

(10) Human-specific paralogs of SRGAP2 induce neotenic features of microglia structural and functional maturationbioRxivSeptember 16, 2024Carlos Diaz-Salazar, Marine Krzisch, Juyoun Yoo, Patricia R. Nano, Aparna Bhaduri, Rudolf Jaenisch, Franck Polleux,Life Technologies,PHC2115 VEGF-121,Life Technologies,PHC9394 M-CSF,Life Technologies,PHC2015 IL-3,Life Technologies,PHC0034 Protamine sulfate,Thermo Scientific,J62926.06 Zeocin,Thermo Scientific,R25001 Dulbecco’s Modified

(11) Adenine base editor corrected ADPKD point mutations in hiPSCs and kidney organoidsAdv Biotechnol (Singap)June 11, 2024Jingwen Wang, Yanling Qiu, Lei Zhang, Xinyao Zhou, Sihui Hu, Qianyi Liu, Sisi Yin, Zehong Su, Simiao Liu, Haiying Liu, et al.35050038), 100 ng/mL SCF (Gibco, PHC2111), 100 ng/mL FLT-3 (Gibco, PHC9414), 20 ng/mL IL-3 (Gibco, PHC0034) and 20 ng/mL IL-6 (Gibco, PHC0065). The cells were then transduced with the CytoTune-iPS 2.0 Sendai

(12) An engineered Sox17 induces somatic to neural stem cell fate transitions independently from pluripotency reprogrammingScience advancesAugust 25, 2023Weng M, Hu H, Graus MS, Tan DS, Gao Y, Ren S, Ho DHH, Langer J, Holzner M, Huang Y, et al.PeproTech, no. 300-07), erythropoietin (10 U/ml) (MedChem Express, no. HY-P7164), IL-3 (50 ng/ml; Gibco, no. PHC0034), 1 μM dexamethasone (Sigma-Aldrich, no. D4902), 1% Insulin-Transferrin-Selenium-Ethanolamine (ITS -X)

(13) microRNA-132 regulates gene expression programs involved in microglial homeostasisiScienceJune 16, 2023Hannah Walgrave, Amber Penning, Giorgia Tosoni, Sarah Snoeck, Kristofer Davie, Emma Davis, Leen Wolfs, Annerieke Sierksma, Mayte Mars, Taofeng Bu, et al.Thermo Fisher Scientific), 50 U/ml penicillin/streptomycin (Cat#P4333-100 mL, Sigma-Aldrich), 25 ng/mL Il-3 (Cat#PHC0035, Thermo Fisher Scientific), and 100 ng/mL M-CSF (Cat#PHC9501, Thermo Fisher Scientific), with fresh

(14) Heterozygous expression of the Alzheimer's disease-protective PLCγ2 P522R variant enhances Aβ clearance while preserving synapsesCellular and molecular life sciences : CMLSJuly 27, 2022Solomon S, Sampathkumar NK, Carre I, Mondal M, Chennell G, Vernon AC, Ruepp MD, Mitchell JC.transferred to a T75 flask and maintained in X-VIVO15 (BE02-060F, Lonza) supplemented with 25 ng/mL IL-3 (PHC0031, ThermoFisher), 100 ng/mL M-CSF (300–25, PeproTech), 2 mM Glutamax (35050061, ThermoFisher) and 0.055

(15) Reactive Astrocytes Derived From Human Induced Pluripotent Stem Cells Suppress Oligodendrocyte Precursor Cell DifferentiationFrontiers in Molecular NeuroscienceMay 6, 2022Matthew D. Smith et al.ng/μL, Thermo Fisher, PHC2111), Flt3 (200 ng/μL, Thermo fisher, PHC9411), IL3 (40 ng/μL, Thermo Fisher, PHC0034), and IL6 (40 ng/μL, Thermo Fisher, PHC0065), GlutaMAX (Thermo fisher, 35050-061) overnight as previously

(16) RNA-Seq is not required to determine stable reference genes for qPCR normalizationPLOS Computational BiologyFebruary 28, 2022Nirmal Kumar Sampathkumar et al.were transferred to a T75 flask in X-VIVO15 (BE02-060F, Lonza) medium supplemented with 25 ng/ml IL-3 (PHC0031, ThermoFisher), 2 mM Glutamax (35050061, ThermoFisher), 100 ng/ml M-CSF (300–25, PeproTech), and 0.055

(17) An early cell shape transition drives evolutionary expansion of the human forebrainCellApril 15, 2021Benito-Kwiecinski S, Giandomenico SL, Sutcliffe M, Riis ES, Freire-Pritchett P, Kelava I, Wunderlich S, Martin U, Wray GA, McDole K, et al.μg/ml ascorbic acid (Sigma, A4403), 50 ng/ml SCF (Miltenyi, 130-096-692), 10 ng/ml IL-3 (Invitrogen, PHC0035), 2 U/ml EPO (R&D Systems, 287-TC-500), 40 ng/ml IGF-1 (Miltenyi, 130-093-885), 1 μM Dexamethasone (Sigma,

(18) Phenotypic and Functional Study of Human Plasmacytoid Dendritic CellsCurr ProtocApril 1, 2021Li G, Cheng L, Su L.Systems. Inc. c/o CytoLogistics) TLR9 agonist CpG A (InvivoGen, cat. NO. tlrl-2216 IL-3 (Gibco, cat. NO. PHC0034) RPMI 1640 medium (Gibco, cat. NO. 11875093) Fetal bovine serum (Gibco, cat. NO. 10100147), 96 well

(19) A CX3CR1 Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-cultureStem cell reportsJune 9, 2020Grubman A, Vandekolk TH, Schröder J, Sun G, Hatwell-Humble J, Chan J, Oksanen M, Lehtonen S, Hunt C, Koistinaho JE, et al.130-096-695, Miltenyi Biotec), IL-6 (50 ng/mL, PHC0061, Thermo Fisher Scientific), and IL-3 (10 ng/mL, PHC0031, Thermo Fisher Scientific) and incubated under normoxia. Half the medium was replaced on D5 and D7 as

(20) FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complexeLifeJune 13, 2018Künzel U, Grieve AG, Meng Y, Sieber B, Cowley SA, Freeman M.,HA peptide,Roche,Cat#I2149-.5MG, "Peptide, recombinant protein",M-CSF,Gibco,Cat#PHC9501, "Peptide, recombinant protein",IL-3,Gibco,Cat#PHC0033, "Peptide, recombinant protein",BMP-4,Invitrogen,Cat#PHC9534, "Peptide, recombinant protein",VEGF,PeproTech,Cat#100–20, "Peptide,

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Human IL-3 Recombinant Protein from Thermo Fisher Scientific

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Human IL-3 Recombinant Protein from Thermo Fisher Scientific

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