Mouse GM-CSF Recombinant Protein PMC2013 from Thermo Fisher Scientific

Product Specs
ItemMouse GM-CSF Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPMC2013
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size1 mg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Novel In Vitro Culture of Microglia from Aged Mice: Implications for the Future of Aging Neurobiology ResearchCurr ProtocAugust 20, 2025Katie L. Reagin, Rae-Ling Lee, Kristen E. FunkGM‐CSF Add 50 µl of 5 µg/ml granulocyte‐macrophage colony‐stimulating factor (GM‐CSF; Gibco, cat. no. PMC2014) to 50‐ml aliquots of microglia growth medium (see recipe) to working concentration of 5 ng/ml. Store

(2) A novel cationic liposome-formulated toll like receptor (TLR) 7/8 agonist enhances the efficacy of a vaccine against fentanyl toxicityJournal of Controlled ReleaseAugust 10, 2025Fatima A. Hamid, Nguyet-Minh Nguyen Nguyen Le, Daihyun Song, Hardik Amin, Linda Hicks, Sophia Bird, Karthik Siram, Brooke Hoppe, Borries Demeler, Jay T. Evans, et al.DC cells were maintained in Alpha minimum essential medium with 5 ng/mL murine GM-CSF (ThermoFisher PMC2015), and 20% fetal bovine serum. Cell activation. Macrophages and DC cells were plated at 0.7 × 106 and

(3) A scalable platform for EPSC-Induced MSC extracellular vesicles with therapeutic potentialStem Cell Res TherAugust 5, 2025Shixin Gong, Nan Li, Qinqing Peng, Feng Wang, Rulong Du, Boyang Zhang, Jian Wang, Le Han, Yu Zhang, Zemin Ning, et al.culturing in IMEM supplemented with 10% fetal bovine serum and 50 ng/mL GM-CSF (Thermo Fisher Scientific, PMC2011) for 7 days. To assess the pro-inflammatory effects of EVs, BMDMs were treated with iMSC-EV (5E8 particles/mL),

(4) AAV1.NT3 gene therapy mitigates the severity of autoimmune encephalomyelitis in the mouse model for multiple sclerosisGene TherapyJuly 1, 2025Lingying Tong, Burcak Ozes, Kyle Moss, Morgan Myers, Zayed Attia, Tatyana A. Vetter, Bruce D. Trapp, Zarife Sahenk35050061), 10 ng/mL recombinant mouse granulocyte macrophages colony-stimulating factor (ThermoFisher PMC2016), 1% penicillin-streptomycin amphotericin B (ThermoFisher R01510) and 50 µM 2-mercaptoethanol (ThermoFisher

(5) Immunomodulatory effects of nanoparticles on dendritic cells in a model of allergic contact dermatitis: importance of PD-L2 expressionScientific reportsSeptember 25, 2023Wong Lau A, Perez Pineda J, DeLouise LA.pyruvate (Gibco Cat# 11360-070), 50 µM β-ME (Gibco Cat# 31350-010), and 20 ng/mL GM-CSF (Gibco Cat# PMC2011). A flow cytometry panel was defined (Table S1) to characterize the development of BMDCs over time.

(6) Immunomodulatory Effects of Nanoparticles on Dendritic Cells in a Model of Allergic Contact Dermatitis - Importance of PD-L2 ExpressionResearch SquareJuly 13, 2023Angela Wong Lau, Jessica Perez Pineda, Lisa A. DeLouisepyruvate (Gibco Cat# 11360–070), 50 uM β-ME (Gibco Cat# 31350–010), and 20 ng/mL GM-CSF (Gibco Cat# PMC2011). A flow cytometry panel was defined (Table. S1) to characterize the development of BMDCs over time.

(7) Peptide-siRNA nanoparticles targeting NF-κB p50 mitigate experimental abdominal aortic aneurysm progression and ruptureBiomater AdvAugust 1, 2022Yan H, Hu Y, Akk A, Wickline SA, Pan H, Pham CTN.containing recombinant murine granulocyte-macrophage colony stimulating factor (GM-CSF) (10 μg/mL, Cat# PMC2015, Thermo Fisher Scientific, Waltham, MA, USA) for 7 days at 37°C. Cultured cells were plated in 12-well

(8) Dendritic peptide-conjugated polymeric nanovectors for non-toxic delivery of plasmid DNA and enhanced non-viral transfection of immune cellsiScienceJuly 15, 2022Sijia Yi, Sun-Young Kim, Michael P. Vincent, Simseok A. Yuk, Sharan Bobbala, Fanfan Du, Evan Alexander Scott,Thermo Fisher Scientific,10082147 2-Mercaptoethanol,Sigma-Aldrich,516732 GM-CSF Recombinant Protein,Thermo Fisher Scientific,PMC2016 IL-4 Recombinant Protein,Thermo Fisher Scientific,RP-8666 L-glutamine,Sigma-Aldrich,59202C GelRed®

(9) Enhanced immunogenic potential of cancer immunotherapy antibodies in human IgG1 transgenic micemAbsJanuary 1, 2022Egli J, Heiler S, Weber F, Steiner G, Schwandt T, Bray-French K, Klein C, Fenn S, Lotz GP, Opolka-Hoffmann E, et al.15140-122), 10% FBS (Seradigm; Cat: 97068-085), and containing 20 ng/ml recombinant mouse GM-CSF (Gibco; Cat: PMC2016). The medium was replenished on day 3 and 6 and the cultures were harvested on day 9 by pipetting. This

(10) Inflammation-induced PELP1 expression promotes tumorigenesis by activating GM-CSF paracrine secretion in the tumor microenvironmentThe Journal of biological chemistryJanuary 1, 2022Vuttaradhi VK, Ezhil I, Ramani D, Kanumuri R, Raghavan S, Balasubramanian V, Saravanan R, Kanakarajan A, Joseph LD, Pitani RS, et al.Calbiochem, TNF-α from Peprotech, Ptx from MP Biomedicals, mouse recombinant GM-CSF protein (catalog no.: PMC2014) from Thermo Fisher Scientific, and STAT3 inhibitor S31-201 (catalog no.: sc-204304) from Santa Cruz Biotechnology

(11) A Straightforward Hypoxic Cell Culture Method Suitable for Standard IncubatorsMethods and ProtocolsApril 8, 2021Svea Matthiesen, Rico Jahnke, Michael R. Knittlercolony-stimulating factor), recombinant protein (Thermo Fisher Scientific, Schwerte, Germany, Cat. No.: PMC2011);Na2HPO4 (sodium hydrogen phosphate) (Roth, Karlsruhe, Germany, Cat. No.: 4984.1);NaCl (sodium chloride)

(12) Generation and utilization of a HEK-293T murine GM-CSF expressing cell linePloS oneJanuary 1, 2021Robinson EK, Covarrubias S, Zhou S, Carpenter S.L929 cells, 5–10% supernatant from the mGM-CSF 293T cell line, or 10–25 ng/ml of recombinant mGM-CSF [PMC2015, Thermo Fisher]. Media was replaced on day 3 and every 2 days henceforward. Cells were scraped and moved

(13) Commensal Microbiota Modulation of Natural Resistance to Virus InfectionCellNovember 25, 2020Stefan KL, Kim MV, Iwasaki A, Kasper DL.Paraformaldehyde solution 4% in PBS,sc-281692,Santa Cruz Biotechnology GM-CSF Recombinant Mouse Protein,ThermoFisher Scientific,PMC2013 ACK Red Blood Cell Lysing Buffer,Gibco,A1049201 Brucella 5% SB HEMIN VIT K1 plates,"BBL, Becton, Dickinson

(14) Small-Molecule Inhibitor of 8-Oxoguanine DNA Glycosylase 1 Regulates Inflammatory Responses during Pseudomonas aeruginosa InfectionJournal of immunology (Baltimore, Md. : 1950)October 15, 2020Qin S, Lin P, Wu Q, Pu Q, Zhou C, Wang B, Gao P, Wang Z, Gao A, Overby M, et al.macrophages by adding recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (PMC2011, Thermo Fisher, Waltham, MA, USA) in the medium. Alveolar lavage fluid Murine (MH-S) cells and J744.1A

(15) Induction of WNT16 via Peptide-mRNA Nanoparticle-Based Delivery Maintains Cartilage HomeostasisPharmaceuticsJanuary 17, 2020Yan H, Hu Y, Akk A, Rai MF, Pan H, Wickline SA, Pham CTN.containing recombinant murine granulocyte-macrophage colony stimulating factor (GM-CSF) (10 µg/mL, Cat# PMC2015, Thermo Fisher Scientific, Waltham, MA, USA) for 7 days at 37 ◦ C. Cultured cells were plated in 12-well

(16) Killed Propionibacterium acnes enhances immunogenicity and tumor growth control of a dendritic-tumor cell hybrid vaccine in a murine melanoma modelPLOS ONEOctober 9, 2018Mayari E. Ishimura, Daniela Teixeira, Gabriela da P. Silveira, Mônica Gambero, Gabriel A. C. Gama, Bruna S. O. Pimenta, Elaine G. Rodrigues, Ieda M. Longo-MaugériDulbecco’s Modified Eagle’s Medium (DMEM) (Gibco-12100046) supplemented with 20 ng of GM-CSF/mL (Gibco-PMC2013), 10% fetal bovine serum (Gibco12657), 1 mM sodium pyruvate (Gibco-11360070), a 1% vitamin solution

(17) Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70Oncology lettersOctober 1, 2018Zhang F, Zhu Y, Fan G, Hu S.RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) with 10% fetal calf serum. rmGM-CSF (10 ng/ml; cat no. PMC2015; Thermo Fisher Scientific, Inc.) and rmIL-4 (1 ng/ml; cat no. RMIL4; Thermo Fisher Scientific, Inc.)

(18) Cancer-Associated Fibroblasts Neutralize the Anti-tumor Effect of CSF1 Receptor Blockade by Inducing PMN-MDSC Infiltration of TumorsCancer cellNovember 13, 2017Kumar V, Donthireddy L, Marvel D, Condamine T, Wang F, Lavilla-Alonso S, Hashimoto A, Vonteddu P, Behera R, Goins MA, et al.mouse,BioLegend,Cat#573702 CXCL-8/IL-8 recombinant human,R&D Systems,Cat#208-IL-010/CF GM-CSF recombinant mouse,Invitrogen,Cat#PMC2013 DAPI,Life Technologies,Cat#D1306 Critical commercial assays,Critical commercial assays,Critical commercial

(19) ErbB4 signaling stimulates pro-inflammatory macrophage apoptosis and limits colonic inflammationCell death & diseaseFebruary 23, 2017Schumacher MA, Hedl M, Abraham C, Bernard JK, Lozano PR, Hsieh JJ, Almohazey D, Bucar EB, Punit S, Dempsey PJ, et al.generate BMDM as previously described,52, 53, 54 or 20 ng/ml GM-CSF (Thermo Scientific, Waltham, MA, USA, PMC2015) to generate BMDC as previously described.55 For BMDMs, adherent cells were washed at 3 days and re-fed

(20) Age-dependent divergent effects of OX40L treatment on the development of diabetes in NOD miceAutoimmunityAugust 1, 2016Haddad CS, Bhattacharya P, Alharshawi K, Marinelarena A, Kumar P, El-Sayed O, Elshabrawy HA, Epstein AL, Prabhakar BS.200 mg/dl was considered hyperglycemic (29–31). Cytokines and antibodies Recombinant mouse GM-CSF (PMC2013) and CellTrace Violet (C34557) were purchased from Invitrogen (Carlsbad, USA). Recombinant mouse TGF-β

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Mouse GM-CSF Recombinant Protein from Thermo Fisher Scientific

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Mouse GM-CSF Recombinant Protein from Thermo Fisher Scientific

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