Human IFN-gamma Recombinant Protein PHC4033 from Thermo Fisher Scientific

Product Specs
ItemHuman IFN-gamma Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHC4033
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size1 mg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Hypoxia-Induced Metabolic and Apoptotic Reprogramming Enhances Immunomodulation in Wharton’s Jelly Mesenchymal Stem CellsiScienceApril 17, 2026Mohini Mendiratta, Meenakshi Mendiratta, Sujata Mohanty, Hridayesh Prakash, Lakshay Malhotra, Sandeep Rai, Vijaya Sarangathem, Sabyasachi Bandyopadhyay, GuruRao Hariprasad, Ritu Gupta, et al.Lipopolysaccharides from Salmonella enterica serotype typhimurium,Sigma-Aldrich,Cat. # L6143 Human IFN-gamma Recombinant Protein,Thermo Fisher Scientific,Cat. # PHC4031 Trichloroacetic acid,HiMedia,Cat. # GRM6274 Ehrlich’s reagent,HiMedia,Cat. # R005 "pHrodoTM Red,

(2) Comparative Analysis of Red Onion-Derived Exosome-Like Nanovesicles and Extract Reveals Sustained Immunomodulatory Effects in LPS/IFN-γ-Stimulated MicrogliaMol NeurobiolMarch 30, 2026Sedra Alhelwani, Nurdan Sena Degirmenci, Cihan Mehmet Altıntaş, Mehtap Aydın, Fikrettin Şahin, Mehmet Şenel, Sevim Işık(Thermo Fisher, R0441) for 10 min then 1 µg/mL of LPS (Sigma-Aldrich, L4391-1MG) and 0.01 µg/mL of IFN-γ (Gibco, PHC4031) for either 4 h or 48 h. After the treatment period, the old medium was replaced with fresh medium,

(3) High‐Content SRS Imaging Unveils Altered Cholesterol Metabolism in Ovarian Cancers Under CAR‐T TreatmentAdvanced ScienceFebruary 15, 2026Chinmayee V. Prabhu Dessai, Zhuoying Huang, Haonan Lin, Guangrui Ding, Hongjian He, Menna Siddiqui, Meng Zhang, Wilson Wong, Ji‐Xin ChengRepository), 10 ng/mL TNF‐𝛼 (Thermo Fisher Scientific, PHC3011), 5 ng/mL IFN‐𝛾 (Thermo Fisher Scientific, PHC4033) respectively for 24 h. The cells were then fixed with a 10% formalin solution before imaging. For the

(4) Tissue-resident macrophage survival depends on mitochondrial function regulated by SerpinB2 in chronic inflammationNat CommunFebruary 12, 2026Sathish Babu Vasamsetti, Samreen Sadaf, Mohammad A. Uddin, Jixing Shen, Ebin Johny, Awishi Mondal, Jonathan Florentin, Liqun Lei, Aleef Mannan, Krithika Sudhakar Rao, et al.bone marrow cells of C57BL/6 mice were cultured and treated with 250 milliunits of IFN-γ (Invitrogen, #PHC4031) or various chemicals such as palmitate (Acros Organics, #416700050), cycloheximide (Alfa Aesar, #J6690103),

(5) ERAP1 Allotypes 2 and 10 Differentially Regulate the Immunopeptidome of MelanocytesImmunologyFebruary 1, 2026Martha Nikopaschou, Martina Samiotaki, Anna Kannavou, Nikolaos V. Angelis, Ourania Tsitsilonis, George Panayotou, Efstratios Stratikos(100,000 cells/well), and 24 h later, they were treated with human recombinant interferon‐γ (IFN‐γ, Gibco, PHC4033) at concentrations ranging from 0 to 50 ng/mL for another 24 h. Cells were subsequently detached and

(6) MHC1-TIP enables single-tube multimodal immunopeptidome profiling and uncovers intratumoral heterogeneity in antigen presentationCommunications BiologyJanuary 22, 2026Mayukha Bathini, Diana Bocaniciu, Fraser D. Johnson, Rick C. P. de Jong, Fengchao Yu, Vincenzo Davide Aloi, Marije C. Kuiken, Janniek R. Mors, Lisanne Giebel, Julien Champagne, et al.(240-B-002, Bio-Techne) for 18 h. For interferon stimulation, the cells were treated with a range of IFNy (PHC4031, GIBCO) doses from 5 ng/mL to 100 ng/mL for 24 h. JY cells were cultured in Roswell Park Memorial Institute

(7) Myeloid cells mediate interferon-driven resistance to immunotherapy in advanced renal cell carcinomaImmunityNovember 11, 2025Kevin Bi, Soki Kashima, Sabrina Y. Camp, Kevin Meli, Eddy Saad, Breanna M. Titchen, Chris Labaki, Ziad Bakouny, Erica M. Pimenta, Jihye Park, et al.unstimulated (control) or primed with recombinant human IFN-γ (10 ng/mL) (Thermo Fisher Scientific, #PHC4031) for 24 h. Both unstimulated and IFN-γ-stimulated macrophages were subsequently used in the killing

(8) Differential Phase Contrast Imaging to Predict MSC Immune FunctionAdvanced Healthcare MaterialsNovember 1, 2025Kejie Rui, Priyanka Priyadarshani, Adrian Ross Liversage, Carlos Munoz, Steven Stice, Luke J. Mortensenreplaced in each well with culture medium containing 0, 50, 100ng mL−1 IFN‐𝛾 (ThermoFisher Scientific, PHC4031) for additional licensing according to experimental design. Then, 100µL conditioned media from each

(9) Immunoproteasome remodeling in senescing human macrophages reveals the loss of PA28αβ capping as a hallmark of immunosenescenceCommunications BiologySeptember 26, 2025Francesca Monittola, Sofia Masini, Mariele Montanari, Maria Gemma Nasoni, Marzia Bianchi, Rita De Matteis, Anastasia Ricci, Daniela Ligi, Francesca Luchetti, Barbara Canonico, et al.MDMs were stimulated with 100 U/ml recombinant human interferon-γ (Gibco, Thermo Fisher Scientific, #PHC4031) for 24 h. Animal model C57BL/6 (B6) and BTBR male mice were reared in the animal shelter of the Department

(10) MHC1-TIP enables single-tube multimodal immunopeptidome profiling and uncovers intratumoral heterogeneity in antigen presentationbioRxivJuly 23, 2025Mayukha Bathini, Diana Bocaniciu, Fraser D. Johnson, Rick C.P. de Jong, Fengchao Yu, Vincenzo Davide Aloi, Marije C. Kuiken, Janniek R. Mors, Lisanne Giebel, Julien Champagne, et al.Bio-Techne) for 18 hours. For interferon stimulation, the cells were treated with a range of IFNy (PHC4031, GIBCO) doses from 5 ng/mL to 100 ng/mL for 24 hours. Flow cytometry A375 cells were washed with PBS

(11) IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cellsOncology ReportsApril 29, 2025Nela Friedlová, Lucie Bortlíková, Lenka Dosedělová, Lukáš Uhrík, Ted R. Hupp, Lenka Hernychová, Bořivoj Vojtěšek, Marta Nekulováto evaluate the effect of IFNγ, the cells were treated with human IFNγ recombinant protein (cat. no. PHC4031; Gibco™; Thermo Fisher Scientific, Inc.) at a concentration of 100 ng/ml for 24 h. SiHa cell line exhibits

(12) Reversible downregulation of HLA class I in adenoid cystic carcinomaJ Immunother CancerApril 20, 2025Annie Li, Bianca L Gonda, Elizabeth M Codd, Adam von Paternos, Dawn R Mitchell, Markus D Herrmann, Prinjali Kalyan, Samantha E Flynn, Thuc Q Dzu, Chengzhuo Gao, et al.(HY-12885B MedChemExpress), 10 µg/mL of pembrolizumab (HY-P9902 MedChemExpress), or 5 ng/mL of IFN-γ (PHC4031, Fisher Scientific). After 48 hours, slices were fixed in 10% neutral buffered formalin for 1 hour/mm

(13) Mimicry of molecular glues using dual covalent chimerasNat CommunMarch 24, 2025Eden Kapcan, Karolina Krygier, Maya da Luz, Nickolas J. Serniuck, Ali Zhang, Jonathan Bramson, Anthony F. Rullogiven by Dr. John Valliant (McMaster University, Canada). IFN-γ was purchased from Fischer Scientific (PHC4031). Ultra-low IgG FBS was purchased from Fischer Scientific (A3381901). DiD cell dye was purchased from

(14) Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade TherapyCancer Immunology ResearchMarch 4, 2025Jiazheng Sun, Pin Wang, Ziying Yi, Yushen Wu, Yuxian Wei, Huiying Fang, Daqiang Song, Yuru Chen, Huimin Du, Jing Huang, et al.transfected with shNC and shWNT7A were treated with either Human IFN-gamma Recombinant Protein (Gibco PHC4033) or Mouse IFN-gamma Recombinant Protein (Gibco PMC4034) at a concentration of 10 ng/mL for 24 hours.

(15) TFIIH kinase CDK7 drives cell proliferation through a common core transcription factor networkScience advancesFebruary 28, 2025Jones T, Feng J, Luyties O, Cozzolino K, Sanford L, Rimel JK, Ebmeier CC, Shelby GS, Watts LP, Rodino J, et al.the cells for a 30-min preincubation, followed by the addition of IFN-γ (1 μg/ml; Life Technologies PHC4031) or 1× PBS. After 45 min ±IFN-γ, the medium was aspirated from the plate and the cells were washed three

(16) Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndromeeLifeFebruary 10, 2025Cozzolino KA, Sanford L, Hunter S, Molison K, Erickson B, Courvan MCS, Jones T, Ajit D, Galbraith MD, Espinosa JM, et al.seeded and allowed to recover for 24 hr prior to being treated with 10 ng/ml interferon gamma (Gibco PHC4031) or vehicle (40 mM Tris pH 7.4). Total RNA was isolated using TRizol (Invitrogen, 15596026) according

(17) Combined PARP14 inhibition and PD-1 blockade promotes cytotoxic T cell quiescence and modulates macrophage polarization in relapsed melanomaJ Immunother CancerJanuary 27, 2025Rotem Leshem, Kieran Neil Sefton, Chun Wai Wong, I-Hsuan Lin, Dervla Tamara Isaac, Mario Niepel, Adam Hurlstoneintervention which we now present below. Materials and methods Reagents Recombinant human IFNγ (PHC4031, Gibco) and recombinant mouse IFNγ (PMC4031, Gibco). PARP14 inhibitor RBN0127597 and PARP14 proteolysis

(18) Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3'-endsbioRxiv : the preprint server for biologyJanuary 8, 2025Luyties O, Sanford L, Rodino J, Nagel M, Jones T, Rimel JK, Ebmeier CC, Shelby GS, Cozzolino K, Brennan F, et al.added to the conditioned media and mixed thoroughly. At t = 30 minutes, 1μg/mL IFNγ (Life Technologies PHC4031; 1000x stock) or 1x phosphate-buffered saline (PBS) was added to the conditioned media and mixed thoroughly.

(19) Modulation of Mitochondrial Dynamics by the Angiotensin System in Dopaminergic Neurons and Microgliaaging and diseaseOctober 31, 2024Aloia Quijano, Ana I. Rodriguez-Perez, María Alicia Costa-Besada, Andrea Lopez-Lopez, María J. Guerra, Jose Luis Labandeira-Garcia, Rita ValenzuelaA9525, Sigma) for 6 h. HMC3 human microglial cells were treated with interferon-gamma (IFNγ; 100ng/ml, PHC4031, Gibco) for 24 h. to induce microglial activation. To test the effect of activation of the RAS protective

(20) Microglia morphological response to mesenchymal stromal cell extracellular vesicles demonstrates EV therapeutic potential for modulating neuroinflammationJournal of Biological EngineeringOctober 17, 2024Kanupriya R. Daga, Andrew M. Larey, Maria G. Morfin, Kailin Chen, Sara Bitarafan, Jana M. Carpenter, Hannah M. Hynds, Kelly M. Hines, Levi B. Wood, Ross A. Markleinstimulated (+ CTL) using half media change with a cytokine cocktail of 5 ng/mL IFN- ү (Gibco, Cat# PHC4033) + 5 ng/mL TNF-α (Sino Biological, Cat#10602-HNAE). Treatment groups received an additional 10 µL of

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Human IFN-gamma Recombinant Protein from Thermo Fisher Scientific

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Human IFN-gamma Recombinant Protein from Thermo Fisher Scientific

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