Human IL-2 Recombinant Protein PHC0027 from Thermo Fisher Scientific

Product Specs
ItemHuman IL-2 Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHC0027
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size40 µg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Ex vivo cytokine responses and metabolic reprogramming are associated with γδ T cell differentiation in Plasmodium falciparum placental malariaFront ImmunolApril 16, 2026Chris Marco Nana Mbianda, Bodin Darcisse Kwanou Tchakounte, Bernard Marie Bitye Zambo, Joséphine Ntankheuh Tchinkou, Derrick Atchombat Nyasse, Balotin Fogang, Rafael José Argüello, Lawrence Ayong, Rosette Megnekoumedium (Gibco, cat# 11020021, USA), supplemented with 100 IU/mL rhIL-2 (Thermo Fisher Scientific, cat# PHC0021, USA), 10% FBS (Gibco, cat# 10082147, USA), 1% Penicillin-Streptomycin (Gibco Thermo Fisher Scientific,

(2) Aiolos and Eos drive distinct human TH17 functional statesCell Mol Life SciApril 1, 2026Silvia Cerboni, Leila Mohammadnezhad, Komal Agrawal, Ramachandramouli Budida, Ilenia Papa, Silvia Gaggero, Joel Dulong, Anna Cole, Anja Mezger, Bingyu Yan, et al.heat-inactivated human serum (SIGMA-Aldrich#H3667) (called ‘complete medium’ here). 500 U/ml IL-2 (Thermofisher# PHC0026) was added to the culture medium for long-term cultures. Single TH17 cell clones were cell sorted in

(3) Thymosin α1-induced secretion of the IL-15/RA complex by THP-1-derived dendritic cells restrains HIV latency in vitroVirulenceMarch 13, 2026Chaoyu Chen, Jingna Xun, Jiangrong Wang, Renfang Zhang, Tangkai Qi, Li Liu, Xinyu Zhang, Zichen Song, Yinzhong Shen, Hongzhou Lu, et al.lower plate. The final concentrations of 1 µM SAHA (MCE, #HY-10221) and 10 ng/mL IL-2 (Invitrogen, #PHC0026) were added to both Tα1-treated experimental group and blank control group for 48 h. IL-2 sustained

(4) CD38 degrades MAVS through mitophagy to inhibit type I interferon secretion in nasopharyngeal carcinoma cells and impairs CD8+T cell-mediated anti-tumor immunityNature CommunicationsFebruary 9, 2026Lin Liang, Wentao Li, Siyi Liu, Qian He, Feng Zeng, Jiaying Cao, Yan Lei, Yanling Li, Yanhong Zhousystem was further augmented with interleukin containing 15 ng/mL recombinant human IL-2 (Catalog No. PHC0026, Invitrogen), 5 ng/mL IL-7 (Catalog No. PHC0073, Invitrogen), and 10 ng/mL IL-15 (Catalog No. PHC9151,

(5) Peripheral blood mononuclear cell (PBMC)- based functional evaluation of human T cell response to suppressive cells and immune-oncology therapeuticsMethods in Cell BiologyJanuary 31, 2026Mahmoud S. Alghamri, Brandon L. McClellan, Kaushik Banerjee, Jorge A. Peña Agudelo, Pedro R. Lowenstein, Maria G. CastroDynabead Human T-Activator CD3/CD28 (Gibco; 11131D) Human IL-2 Recombinant Protein (rIL-2; Gibco; PHC0026) GolgiPlug Protein Transport Inhibitor (Containing Brefeldin A) (BD; 555029) GolgiStop Protein Transport Inhibitor (Containing Monensin

(6) Dysregulation of apolipoprotein o reprograms CCR7+CD4+T cell fate in primary autoimmune thrombocytopeniaiScienceNovember 21, 2025Tengda Li, Xiang Li, He Huangproteins","Chemicals, peptides, and recombinant proteins" ,, Human IL-2 Recombinant Protein,Thermo Fisher Scientific,PHC0021 Antimycin A,Sigma,1397-94-0 AICAR,Sigma,2627-69-2 ,, Critical commercial assays,Critical commercial

(7) Protocol for the biofabrication of immunocompetent tumor-on-chip models from patient solid tumors for assessment of anticancer therapiesSTAR ProtocolsSeptember 19, 2025Martin Nurmik, Ségolène Ladaigue, Isabella Hofer, Auriane Debache, Irina Veith, Fatima Mechta-Grigoriou, Stephanie Descroix, Gerard Zalcman, Maria Carla Parrinisupernatant.19.Resuspend the cells in requisite amount of Medium A, supplemented with 30 ng/mL of rIL-2 (Gibco, #PHC0021), to obtain approximately 5000 CD8+ TILs cells per 100 μL of media.20.Seed 200 μL of CD8+ cell suspension

(8) Bioengineering a Patient‐Derived Vascularized Lung Tumor‐on‐Chip Model to Decipher Immunomodulation by the EndotheliumAdvanced Healthcare MaterialsSeptember 1, 2025Christine Lansche, Ségolène Ladaigue, Giacomo Gropplero, Nicolas Zimmermann, Martin Nurmik, Irina Veith, Manh‐Louis Nguyen, Solenn Brosseau, Nicolas Poté, Pierre Mordant, et al.penicillin/streptomycin solution, 1% sodium pyruvate (Thermo‐Fisher Scientific, 11‐360‐070), 30 ng mL−1 of rIL‐2 (Gibco, #PHC0021). Primary lung CAFs were cultured into standard culture dishes DMEM/high glucose (Cytiva, SH30243.01)

(9) A 3D Tumor Spheroid Model with Robust T Cell Infiltration for Evaluating Immune Cell EngagersiScienceAugust 15, 2025Hin Ching Lo, Hana Choi, Kevin Kolahi, Samantha Rodriguez, Leiru Gonzalez, Felix Chu, Aditee Shrotre, Xi Zhao, Aloma L. D’Souza, Ron Ferrando, et al.12352200 DNase I,Invitrogen,Cat# 18068015 RPMI-1640 media,Gibco,Cat# 11875093 IL2,ThermoFisher,Cat# PHC0021 Pembrolizumab,Selleck Chem,Cat# A2005 EpCAM-CD3 bispecific antibody,Creative Biolabs,Cat# BSSG-079 DAPI,Akoya,Cat#

(10) Immune targeting of triple-negative breast cancer through a clinically actionable STING agonist-CAR T cell platformCell Reports MedicineJuly 15, 2025Yuqing Zhang, Zehua Li, Jessica Ritter, Elliott J. Brea, Navin R. Mahadevan, Deborah A. Dillon, Sung-Rye Park, Eric Minwei Liu, Michael Y. Tolstorukov, William E. McGourty, et al.314042-01-8 "T cell TransAct™, human",Miltenyi Biotec,Cat# 130-111-160 Human IL-2,Thermo Fisher Scientific,Cat# PHC0021 Human IL-7,Thermo Fisher Scientific,Cat# PHC0075 Human IL-15,Thermo Fisher Scientific,Cat# PHC9154 ,, Critical

(11) IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cellsOncology ReportsApril 29, 2025Nela Friedlová, Lucie Bortlíková, Lenka Dosedělová, Lukáš Uhrík, Ted R. Hupp, Lenka Hernychová, Bořivoj Vojtěšek, Marta NekulováScientific, Inc.), 0.02 mM folic acid (cat. no. F8758; MilliporeSigma), 200 U/ml recombinant IL-2 (cat. no. PHC0026; Gibco; Thermo Fisher Scientific, Inc.), 25% FBS and penicillin-streptomycin at 37°C with 5% CO2. The

(12) Development of a Mechanism of Action-Reflective Cell-Based Reporter Gene Assay for Measuring Bioactivities of Therapeutic Glucagon-like Peptide-2 AnaloguesMoleculesApril 25, 2025Xiaoming Zhang, Chunyan Li, Zhe Deng, Chenggang Liang, Jing Lifetal bovine serum (FBS), 200 µg/mL zeocin, and 3 µg/mL puromycin. Both antibiotics, recombinant human Interleukin-2 (IL-2, PHC0027), IL-4 (200-04), IL-6 (200-06), and mouse IgG isotype control were purchased from Thermo Fisher Scientific

(13) A Reporter Gene Assay for Measuring the Biological Activity of PEGylated Recombinant Human Growth HormoneMoleculesFebruary 3, 2025Shaowang Hu, Xiaoming Zhang, Yi Li, Jing Li, Yingwu Wang, Chenggang Liangµg/mL blasticidin S. All three antibiotics, lipofectamine 3000, recombinant human Interleukin-2 (IL-2, PHC0027), IL-4 (200-04), EGF (PHG0311L), and IGF-1 (RP-10931) were purchased from Thermo Fisher Scientific (Waltham,

(14) Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indicationsScientific ReportsJanuary 31, 2025Colin D. Paul, Chris Yankaskas, Pradip Shahi Thakuri, Brittany Balhouse, Shyanne Salen, Amber Bullock, Sylvia Beam, Anthony Chatman, Sybelle Djikeng, Xiaoyu Jenny Yang, et al.Scientific, cat# 26050-088) + 500 U/ml Gibco recombinant human (rh) IL-2 (Thermo Fisher Scientific, cat# PHC0021). For primary NK cells, human leukopaks were procured from the San Diego Blood Bank and processed using

(15) Dynamics of tissue repair regulatory T cells and damage in acute Trypanosoma cruzi infectionPLoS pathogensJanuary 1, 2025Boccardo S, Rodriguez C, Gimenez CMS, Araujo Furlan CL, Abrate CP, Almada L, Saldivia Concepción MA, Skewes-Cox P, Rao SPS, Mukdsi JH, et al.detailed above. When indicated, i.p. injections also contained 1 μg of recombinant human IL-2 (Gibco, Cat# PHC0021). PBS was used as vehicle. For treatments with neutralizing antibodies, mice received i.p. injections

(16) Extracellular vesicles released by cancer-associated fibroblast-induced myeloid-derived suppressor cells inhibit T-cell functionOncoImmunologyDecember 31, 2024Carlo P. Ramil, Handan Xiang, Peng Zhang, Aileen Cronin, Lisia Cabral, Zhizhang Yin, Josephine Hai, Huijun Wang, Benjamin Ruprecht, Yanlin Jia, et al.at a ratio of 2:1 (100,000:50,000) T:M in a 96-well plate (Corning #7007) containing IL-2 (Gibco # PHC0026), CD3/CD28 beads (Thermo #11161D) and filled with complete media to a final volume of 200 µL. Nonproliferating

(17) Promising approach for targeting ROBO1 with CAR NK cells to combat ovarian cancer primary tumor cells and organoidsFuture Science OADecember 31, 2024Yan Zhu, Luanhong Wang, Biyu Jiang, Yini Wang, Qing Wu, Sihua Hong, Xiaojing Wang, Yuancheng Li, Tian Guan, Haoyu Zeng, et al.culturing, besides the regents used in the kits, additional low-dose of human IL-2 Recombinant Protein (PHC0026, Gibco, USA) and NK Inducing Regent (IR0103, iPSyte Biotec, Guangzhou, CN) were added into the medium,

(18) Low intensity mechanical signals promote proliferation in a cell-specific manner: Tailoring a non-drug strategy to enhance biomanufacturing yieldsMechanobiology in MedicineDecember 24, 2024M. Ete Chan, Christopher Ashdown, Lia Strait, Sishir Pasumarthy, Abdullah Hassan, Steven Crimarco, Chanpreet Singh, Vihitaben S. Patel, Gabriel Pagnotti, Omor Khan, et al.Dynabead® CD3/CD28 ([cat. 11141D; Thermo Fisher Scientific, MA) and recombinant interleukin-2 (rIL-2, [cat. PHC0026,10 ng/mL; Thermo Fisher Scientific, MA) for activation. During the initial activation phase, Dynabeads®

(19) Identification of SPP1 + macrophages as an immune suppressor in hepatocellular carcinoma using single-cell and bulk transcriptomicsFrontiers in ImmunologyDecember 3, 2024Han Jin, Woonghee Kim, Meng Yuan, Xiangyu Li, Hong Yang, Mengzhen Li, Mengnan Shi, Hasan Turkez, Mathias Uhlen, Cheng Zhang, et al.supplemented with 10% fetal bovine serum (FBS, F7524, Sigma-Aldrich), 1% P/S (P4333, Sigma-Aldrich), IL-2 (PHC0026, Thermo Fisher), and DynabeadsTM Human T-Activator CD3/CD28 (11161D, Thermo Fisher). 2x106 cells were

(20) Smartphone-driven centrifugal microfluidics for diagnostics in resource limited settingsBiomedical microdevicesOctober 26, 2024Lapins N, Akhtar AS, Banerjee I, Kazemzadeh A, Pinto IF, Russom A.calibration curve of IL-2 were prepared by diluting recombinant human IL-2 (ThermoFisher Scientific, Cat. No. PHC0026) in PBS to obtain the desired range of concentrations. 100 μL of the standard was added to the wells

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Human IL-2 Recombinant Protein from Thermo Fisher Scientific

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Human IL-2 Recombinant Protein from Thermo Fisher Scientific

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