Human IL-1 beta Recombinant Protein PHC0811 from Thermo Fisher Scientific

Product Specs
ItemHuman IL-1 beta Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHC0811
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size100 µg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Unanchored K63-linked polyubiquitin chains: a novel second messenger involved in G protein-coupled receptors early signaling eventsCell Communication and SignalingJanuary 8, 2026Priscilla Doyon, Daniel El-Mortada, Jiunn Roy, Firas El-Mortada, Florence Dô, Simon-Pierre Gravel, Marc J. Servant13-acetate (PMA, P1585) were purchased from Sigma Aldrich; recombinant human Interleukin 1 beta (IL1β, PHC0815) was from ThermoFisher and isopeptidase T/USP5, rabbit (E-320) was obtained from BostonBiochem. Commercial

(2) Use of femtosecond laser generated reactive oxygen species to manage inflammation and enhance wound healing after ocular traumaSci RepJuly 1, 2025Jiashuai Fan, Mikhail Fomovsky, Chao Wang, Mariya Zyablitskaya, Christos Sazeides, Dusan Bogunovic, Sinisa Vukelic1. Interleukin solution laser treatment procedures Recombinant human IL-β protein (Thermo Fisher Scientific, PHC0815) was reconstituted to a concentration of 5 μg/mL using dH2O following the manufacturer’s guidelines.

(3) IL1RAP Expression in Human Atherosclerosis: A Target of Novel Antibodies to Reduce Vascular Inflammation and AdhesionJournal of the American Heart AssociationMay 20, 2025Madelene Lindkvist, Anna Göthlin Eremo, Geena Varghese Paramel, Sheikh Anisul Haque, Camilla Rydberg Millrud, Sara Rattik, Caitríona Grönberg, David Liberg, Allan Sirsjö, Karin FranséneBioscience/Invitrogen/Thermo Fisher Scientific) for 1 hour, followed by treatment with 5 ng/mL mature IL‐1β (PHC0814; Thermo Fisher Scientific), 100 ng/mL IL‐33 (R&D Systems), or 100 ng/mL IL‐36γ (R&D Systems) for 30

(4) The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosiseLifeFebruary 6, 2025Hsieh LT, Hall BS, Newcombe J, Mendum TA, Varela SS, Umrania Y, Deery MJ, Shi WQ, Diaz-Delgado J, Salguero FJ, et al.protein",Human Laminin-511,BioLamina,LN511-0202,5 μg/ml "Peptide, recombinant protein",Human IL-1β,Gibco,PHC0813,100 ng/ml Commercial assay or kit,ImmPACT NovaRED peroxidase substrate kit,Vector Laboratories,SK4805, Commercial

(5) Cuprorivaite microspheres inhibit cuproptosis and oxidative stress in osteoarthritis via Wnt/β-catenin pathwayMaterials today. BioDecember 1, 2024Li B, Jiang T, Wang J, Ge H, Zhang Y, Li T, Wang C, Wang W.experiment procedures To mimic the inflammatory chondrocytes in OA, 10 ng/mL IL-1β recombinant protein (PHC0811, Gibco, USA) was used to incubate cells for 24 h at 37 °C [26]. cuprorivaite microspheres with different

(6) The pro-inflammatory cytokines IL-1β and IL-6 promote upregulation of the ST6GAL1 sialyltransferase in pancreatic cancer cellsThe Journal of biological chemistryOctober 1, 2024Silva AD, Hwang J, Marciel MP, Bellis SL.ng/ml human recombinant EGF (Thermo Fisher, PHG0311), and 5% FBS. Recombinant IL-1β (Thermo Fisher, PHC0811) and IL-6 (Thermo Fisher, 8954SR025CF) were reconstituted in saline containing 0.1% bovine serum albumin (BSA

(7) Synovium friction properties are influenced by proteoglycan contentJournal of biomechanicsSeptember 1, 2024Gangi LR, Pagon AD, Pellicore MJ, Kroupa KR, Murphy LA, Ateshian GA, Hung CT.their respective species: IL-1α (ThermoFisher, PHC0015) for bovine specimens and IL-1β (ThermoFisher, PHC0815) for human samples. Untreated explants (CTL) were cultured in CM for 4 days. For the proinflammatory

(8) Influence of inflammation on the expression of microRNA-140 in extracellular vesicles from 2D and 3D culture models of synovial-membrane-derived stem cellsFrontiers in Bioengineering and BiotechnologyAugust 7, 2024João Pedro Hübbe Pfeifer, Fernanda de Castro Stievani, Célio J. da Costa Fernandes, Gustavo dos Santos Rosa, Emanuel Vitor Pereira Apolonio, Mariana Correa Rossi, Willian Fernando Zambuzzi, Ana Liz Garcia AlvesDMEM-F12/glutamax +10% FBSevfree + 1% antibiotic–antimycotic +10 ng/mL IL-1β (Sylvester et al., 2012) (#PHC0814. Gibco, Grand Island, NY, United States) and 0.25 ng/mL TNFα (Westacott et al., 2000) (#PHC3015L. Gibco,

(9) Peptain-1 blocks ischemia/reperfusion-induced retinal capillary degeneration in miceFrontiers in Cellular NeuroscienceAugust 1, 2024Mi-Hyun Nam, Armaan Dhillon, Rooban B. Nahomi, Noelle L. Carrillo, Clarinda S. Hougen, Ram H. NagarajSan Jose, CA, Cat #285-IF) +TNF-α (Invitrogen, Carlsbad, CA, Cat# PHC3015L) + IL-1β (Invitrogen, Cat #PHC0815)] for 48 h. Cell lysates were prepared with 1X RIPA buffer (Thermo Fisher Scientific, Waltham, MA, Cat#

(10) Disease-associated astrocyte epigenetic memory promotes CNS pathologyNatureMarch 28, 2024Hong-Gyun Lee, Joseph M. Rone, Zhaorong Li, Camilo Faust Akl, Seung Won Shin, Joon-Hyuk Lee, Lucas E. Flausino, Florian Pernin, Chun-Cheih Chao, Kilian L. Kleemann, et al.media was then added into the wells. The cells were treated for 18–24 h with 10 ng ml−1 IL-1β (Gibco, PHC0815) and 5 ng ml−1 TNF (Gibco, PHC3016) in basal media. After the first stimulation, cells were washed 2

(11) Transient inhibition of meniscus cell migration following acute inflammatory challengeJournal of Orthopaedic ResearchSeptember 1, 2023Elisabeth A. Lemmon, Edward D. Bonnevie, Jay M. Patel, Liane M. Miller, Robert L. Mauckrecombinant; R&D Systems catalog #201LB005) or IL1β (human recombinant; ThermoFisher Scientific catalog #PHC0815). Cytokines were introduced at the same time that the monolayer was scratched with a 200 μL pipette

(12) Akkermansia muciniphila and its membrane protein ameliorates intestinal inflammatory stress and promotes epithelial wound healing via CREBH and miR-143/145Journal of biomedical scienceJune 7, 2023Wade H, Pan K, Duan Q, Kaluzny S, Pandey E, Fatumoju L, Saraswathi V, Wu R, Harris EN, Su Q.(IL)-1β treatment was performed under identical conditions and treated with 10 ng/mL human IL-1β (Gibco, PHC0814) for 24 or 48 h. For transfection, 3 × 105 cells were seeded in wells of 6-well plates and grown to

(13) Upregulation of PD-L1 by SARS-CoV-2 promotes immune evasionJournal of medical virologyFebruary 1, 2023Huang HC, Wang SH, Fang GC, Chou WC, Liao CC, Sun CP, Jan JT, Ma HH, Ko HY, Ko YA, et al.Roche). After 6 h, cells were treated with 10 ng/ml TGF‐β (PHG9214; Fisher Scientific), 10 ng/ml IL‐1β (PHC0811; Fisher Scientific), 10 ng/ml IL‐6 (PHC0061; Fisher Scientific), 10 ng/ml EGF (PHG0311, Fisher Scientific),

(14) Selective ablation of P53 in pancreatic beta cells fails to ameliorate glucose metabolism in genetic, dietary and pharmacological models of diabetes mellitusMolecular metabolismJanuary 1, 2023Uhlemeyer C, Müller N, Rieck M, Kuboth J, Schlegel C, Grieß K, Dorweiler TF, Heiduschka S, Eckel J, Roden M, et al.necrosis factor alpha (TNFα, 1000 U/ml, #PMC3014, Thermo Fisher), interleukin 1 beta (IL1β, 50 U/ml, #Phc0814, Thermo Fisher) and interferon gamma (IFNγ, 1000 U/ml for 40 h, #PMC4031, Thermo Fisher); Nutlin-3 (10

(15) Cerebrospinal fluid concentration of complement component 4A is increased in first episode schizophreniaNat CommunNovember 3, 2022Jessica Gracias, Funda Orhan, Elin HÃ¶rbeck, Jessica HolmÃ©n-Larsson, Neda Khanlarkani, Susmita Malwade, Sravan K. Goparaju, Lilly Schwieler, Ä°lknur Åž. Demirel, Ting Fu, et al.days, mature NGN2 neurons were treated with either VEH, 1 ng/mL IL-1β (Thermo Fisher Scientific, cat# PHC0815) or 1 ng/mL IL-6 (Thermo Fisher Scientific, cat# PHC0063), alone or in combination for 24 hours. Following

(16) P300 promotes tumor recurrence by regulating radiation-induced conversion of glioma stem cells to vascular-like cellsNature CommunicationsOctober 19, 2022Sree Deepthi Muthukrishnan, Riki Kawaguchi, Pooja Nair, Rachna Prasad, Yue Qin, Maverick Johnson, Qing Wang, Nathan VanderVeer-Harris, Amy Pham, Alvaro G. Alvarado, et al.at a concentration of 50–100 ng/ml (hFGF7, Peprotech, 100-19-10ug), hIL1B (Thermofisher scientific, PHC0814), hIL23A (R&D systems, 1290-IL-010/CF), hIL33 (Thermofisher Scientific, 34-8539-63), hWNT16B (R&D systems,

(17) Potential Therapeutic Activity of Berberine in Thyroid-Associated Ophthalmopathy: Inhibitory Effects on Tissue Remodeling in Orbital FibroblastsInvestigative ophthalmology & visual scienceSeptember 1, 2022Diao J, Chen X, Mou P, Ma X, Wei R.Eagle's medium (DMEM), fetal bovine serum (FBS), 0.25% trypsin-EDTA, and human IL-1β recombinant protein (PHC0815) were provided by Gibco (Waltham, MA, USA). Mesenchymal stem cell adipogenic differentiation medium

(18) TREM2 macrophages induced by human lipids drive inflammation in acne lesionsScience immunologyJuly 22, 2022Do TH, Ma F, Andrade PR, Teles R, de Andrade Silva BJ, Hu C, Espinoza A, Hsu JE, Cho CS, Kim M, et al.cat. # 554617), IL-4 (20ng/mL) (Peprotech, cat. # 200–04), IL-1β (1 ng/mL) (Thermo Scientific, cat. # PHC0815), squalene (1 mM) (Thermofisher Scientific, cat.# S3626–100ML), cholesterol (0.025 mM, 0.25 mM, 0.5

(19) MicroRNA-210 regulates the metabolic and inflammatory status of primary human astrocytesJournal of neuroinflammationJanuary 6, 2022Kieran NW, Suresh R, Dorion MF, MacDonald A, Blain M, Wen D, Fuh SC, Ryan F, Diaz RJ, Stratton JA, et al.then added into wells. The cells were treated for 24 h with 1 ng/mL interleukin-1beta (IL1b, Gibco, PHC0815) in basal media, glucose-free DMEM/F12 media (ThermoFisher, USA, 11966025), or 1% O2 in normal culture

(20) SPLUNC1: a novel marker of cystic fibrosis exacerbationsThe European respiratory journalNovember 1, 2021Khanal S, Webster M, Niu N, Zielonka J, Nunez M, Chupp G, Slade MD, Cohn L, Sauler M, Gomez JL, et al.human airway epithelial cells, were treated with recombinant human IL-1β (Gibco, Gaithersburg, MD, cat# PHC0811,) or TNF-α (R&D, Minneapolis, MN, cat# 210-TA-005) at 10 ng/ml. Cellular mRNA was extracted for qPCR,

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Human IL-1 beta Recombinant Protein from Thermo Fisher Scientific

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Human IL-1 beta Recombinant Protein from Thermo Fisher Scientific

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