Mouse IFN-gamma Recombinant Protein PMC4033 from Thermo Fisher Scientific

Product Specs
ItemMouse IFN-gamma Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPMC4033
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size1 mg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Host response to bacteria induces a shift towards the enteroendocrine cell lineage in the murine enteroid modelPLOS PathogensMarch 23, 2026Marry Nissan, Adrienne Ranger, Justin J. Meade, Victoria Gillmore, Maija E. Lehn, Liliane Cabral-Fernandes, Derek K. L. Tsang, Scott D. Gray-Owen, Dana J. Philpott, Stephen E. Girardintlrl-c12dap), Tri-DAP (Invivogen, tlrl-tdap), TNF (ThermoFisher, 315-01A-5UG), IFNγ (ThermoFisher, PMC4031), Fluoresbrite YG Carboxylate Microspheres 1.00µm (Polysciences, 15702), DAPT (abcam, ab120633), ISX-9

(2) Estrogen receptor signaling drives immune evasion and immunotherapy resistance in HR+ breast cancerJournal of Clinical InvestigationJanuary 16, 2026José Ángel Palomeque, Gabriel Serra-Mir, Sandra Blasco-Benito, Helena Brunel, Pau Torren-Duran, Iván Pérez-Núñez, Chiara Cannatá, Laura Comerma, Silvia Menendez, Sonia Servitja, et al.at different ratios. Cells were treated with 1 μg/mL mouse IFN-γ (Gibco, Thermo Fisher Scientific, PMC4031) 24 hours prior to coculturing for control and ER experiments. After 96 hours, cell viability was assayed

(3) Biomimetic fractal topography enhances podocyte maturation in vitroNat CommunDecember 15, 2025Chuan Liu, Praful Aggarwal, Karl T. Wagner, Shira Landau, Teng Cui, Xin Song, Laleh Shamaei, Naimeh Rafatian, Yimu Zhao, Sonia Rodriguez-Ramirez, et al.under the proliferative condition at 33 °C with 10 units/mL mouse interferon gamma (Thermo Scientific, PMC4031) supplemented in fresh RPMI 1640 basal media containing HEPES and GlutaMAX (Gibco, #72400120) with 10%

(4) Transitory Schwann Cell Precursor and hybrid states underpin melanoma therapy resistance and metastasisbioRxivJuly 24, 2025Vishaka Gopalan, Chun Wai Wong, Rotem Leshem, Luke Owen, Tuulia Vallius, Yingxiao Shi, Yuhong Jiang, Eva Pérez-Guijarro, Emily Wu, Sung Chin, et al.and the P-value was calculated by the log-rank (Mantel-Cox) test. Reagents Recombinant mouse IFNγ (PMC4031) was purchased from Gibco, mouse TNF-alpha Recombinant Protein (RMTNFAI) was purchased from Thermo Fisher Scientific

(5) GRA12 is a common virulence factor across Toxoplasma gondii strains and mouse subspeciesNat CommunApril 16, 2025Francesca Torelli, Simon Butterworth, Eloise Lockyer, Ana N. Matias, Franziska Hildebrandt, Ok-Ryul Song, Jennifer Pearson-Farr, Moritz Treeckused in the in vivo screen. 7E6 BMDMs were seeded and treated for 24 h with 10 U/ml murine IFNγ (Gibco PMC4031) or left untreated prior to infection. To limit co-infections, 1.4E6 parasites per dish corresponding

(6) Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade TherapyCancer Immunology ResearchMarch 4, 2025Jiazheng Sun, Pin Wang, Ziying Yi, Yushen Wu, Yuxian Wei, Huiying Fang, Daqiang Song, Yuru Chen, Huimin Du, Jing Huang, et al.either Human IFN-gamma Recombinant Protein (Gibco PHC4033) or Mouse IFN-gamma Recombinant Protein (Gibco PMC4034) at a concentration of 10 ng/mL for 24 hours. Following treatment, the cell suspensions were prepared. We

(7) Combined PARP14 inhibition and PD-1 blockade promotes cytotoxic T cell quiescence and modulates macrophage polarization in relapsed melanomaJ Immunother CancerJanuary 27, 2025Rotem Leshem, Kieran Neil Sefton, Chun Wai Wong, I-Hsuan Lin, Dervla Tamara Isaac, Mario Niepel, Adam Hurlstonebelow. Materials and methods Reagents Recombinant human IFNγ (PHC4031, Gibco) and recombinant mouse IFNγ (PMC4031, Gibco). PARP14 inhibitor RBN0127597 and PARP14 proteolysis targeting chimera (PROTAC) RBN012811 (Ribon

(8) CB2 expression in mouse brain: from mapping to regulation in microglia under inflammatory conditionsJournal of NeuroinflammationAugust 19, 2024Wanda Grabon, Anne Ruiz, Nadia Gasmi, Cyril Degletagne, Béatrice Georges, Amor Belmeguenai, Jacques Bodennec, Sylvain Rheims, Guillaume Marcy, Laurent Bezintreated with LPS (from Escherichia coli O55:B5, 100 ng/mL, Sigma #L6529) or with IFNγ (100 ng/mL, Gibco #PMC4031) for the indicated time, and harvested for further RT-qPCR analysis. Blockade of IL-1R was performed

(9) IL-3-driven T cell–basophil crosstalk enhances antitumor immunityCancer Immunology ResearchJuly 2, 2024Jian Wei, Colleen L. Mayberry, Xiaoting Lv, Fangyan Hu, Taushif Khan, Natalie A. Logan, John J. Wilson, John D. Sears, Damien Chaussabel, Chih-Hao Changdescribed (13). Briefly, B16-OVA melanoma cells were pre-treated with 300 units/mL murine IFN-γ (Peprotech, PMC4031) for 48 hours. Then, a single cell suspension was prepared. Target B16-OVA cells were generated by staining

(10) Glutathione-mediated redox regulation in Cryptococcus neoformans impacts virulenceNature MicrobiologyJuly 2, 2024Braydon Black, Leandro Buffoni Roque da Silva, Guanggan Hu, Xianya Qu, Daniel F. Q. Smith, Armando Alcázar Magaña, Linda C. Horianopoulos, Mélissa Caza, Rodgoun Attarian, Leonard J. Foster, et al.plates and incubated at 37 °C in 5% CO2 for 24 h in DMEM supplemented with 100 U ml−1 of IFN-γ (Gibco, PMC4031) to activate. Overnight cultures of WT and gsh2Δ C. neoformans grown in YPD were washed twice with PBS,

(11) HIF-1α activation impairs dendrites formation and elongation in osteocytogenesisHeliyonJune 30, 2024Xiaoyan Li, Jian Zhao, Lei Chen, Xinyi Zhou, Minglong Qiu, Lianfu Deng, Kai Yang, Yaozeng XuBonewald [35]. Cells were proliferated and differentiated under different mediums, with 50 U/ml IFN-γ (#PMC4031, Invitrogen) at 33 °C for proliferation or with 4 mM β-Glycerophosphate (G9422, Sigma) and 50 μg/ml

(12) Interpenetrating network hydrogels for studying the role of matrix viscoelasticity in 3D osteocyte morphogenesis†Biomaterials ScienceFebruary 13, 2024Margherita Bernero, Doris Zauchner, Ralph Müller, Xiao-Hua Qinfetal bovine serum (FBS, Gibco, 10270-106), 50 U mL−1 recombinant mouse interferon-gamma (IFN-γ, Gibco, PMC4031) and 1% antibiotic–antimycotic (Gibco, 15240062)) and cultured at 33 °C and 5% CO2. At 90% confluence,

(13) Production of a p65(fl/fl)/LysMCre mouse model with dysfunctional NF-κB signaling in bone marrow-derived macrophagesInnate immunityNovember 1, 2023Korkaya AK, Fischer J, Peppers A, Crosson SM, Rayamajhi M, Miao EA, Baldwin AS Jr, Bradford JW.3610). The BMDMs were allowed to settle overnight, and were then treated with 150U of IFNγ (Fisher PMC4031) for 6 h, or vehicle control. The media was then removed, cells were washed, and 0.2 µg of LPS was added

(14) A heterotrimeric complex of Toxoplasma proteins promotes parasite survival in interferon gamma-stimulated human cellsPLoS biologyJuly 1, 2023Lockyer EJ, Torelli F, Butterworth S, Song OR, Howell S, Weston A, East P, Treeck M.A1443001) with or without 100 U/ml IFNγ (Human:Bio-Techne, 285-IF-100| Mouse: Thermo Fisher, Gibco PMC4031) for 24 h. Parasite strains were syringe lysed and added to host cells at 40,000 parasites/well. Plates

(15) Targeting ULK1 Decreases IFNγ-Mediated Resistance to Immune Checkpoint InhibitorsMolecular cancer research : MCRApril 1, 2023Fenton SE, Zannikou M, Ilut L, Fischietti M, Ji C, Oku CV, Horvath CM, Le Poole IC, Bosenberg M, Bartom ET, et al.vehicle-control (DMSO), 10μM SBI and/or 2,500 IU/mL IFNγ (Gibco, human IFNγ # PHC4033 and mouse IFNγ # PMC4033). Cell pellets were collected and processed for RNA isolation. To assess the effects of ULK1 drug-targeted

(16) Selective ablation of P53 in pancreatic beta cells fails to ameliorate glucose metabolism in genetic, dietary and pharmacological models of diabetes mellitusMolecular metabolismJanuary 1, 2023Uhlemeyer C, Müller N, Rieck M, Kuboth J, Schlegel C, Grieß K, Dorweiler TF, Heiduschka S, Eckel J, Roden M, et al.interleukin 1 beta (IL1β, 50 U/ml, #Phc0814, Thermo Fisher) and interferon gamma (IFNγ, 1000 U/ml for 40 h, #PMC4031, Thermo Fisher); Nutlin-3 (10 μM for 6 h, #N6287, Sigma-Aldrich); Olaparib (10 μM for 3 h + 16 h (pre-

(17) Combinatorial immunotherapies overcome MYC-driven immune evasion in triple negative breast cancerNature communicationsJune 27, 2022Lee JV, Housley F, Yau C, Nakagawa R, Winkler J, Anttila JM, Munne PM, Savelius M, Houlahan KE, Van de Mark D, et al.#12115-1), IFNβ was used at 1,000 U/mL (PBL Assay Science, #12405-1), IFNγ was used at 100 ng/mL (Gibco, #PMC4031). Statistics for biological experiments Graphs and analysis performed in Prism 9 (v 9.1.0). Two-tailed,

(18) Dual G9A/EZH2 Inhibition Stimulates Antitumor Immune Response in Ovarian High-Grade Serous CarcinomaMolecular cancer therapeuticsApril 1, 2022Spiliopoulou P, Spear S, Mirza H, Garner I, McGarry L, Grundland-Freile F, Cheng Z, Ennis DP, Iyer N, McNamara S, et al.On day 0, the drug library was added, along with 1 ng/mL of mouse IFNγ (Thermo Fisher Scientific, #PMC4031). After 72 hours, supernatant was transferred onto CXCL10 ELISA plates (R&D Systems DY466) using the

(19) Macrophage global metabolomics identifies cholestenone as host/pathogen cometabolite present in human Mycobacterium tuberculosis infectionJournal of Clinical InvestigationFebruary 1, 2022Pallavi Chandra, Héloise Coullon, Mansi Agarwal, Charles W. Goss, Jennifer A. PhilipsSigma) for 24 hours before infection. BMDMs were activated with IFN-γ (Thermo Fisher Scientific, catalog PMC4031) at a concentration of 100 U/mL. Global metabolomics profiling. For global metabolic profiling, BMDMs

(20) Long non‑coding RNA TUG1 sponges microRNA‑9 to protect podocytes from high glucose‑induced apoptosis and mitochondrial dysfunction via SIRT1 upregulationExperimental and Therapeutic MedicineJanuary 24, 2022Min Lei, Guibao Ke, Yan Wang, Dan Luo, Yao Huno. 10099141C; Thermo Fisher Scientific, Inc.) and 10 U/ml-1 recombinant mouse interferon-γ (cat. no. PMC4033; Thermo Fisher Scientific, Inc.) at 33˚C under a humidified atmosphere of 5% CO2 for propagation, as

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Mouse IFN-gamma Recombinant Protein from Thermo Fisher Scientific

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Mouse IFN-gamma Recombinant Protein from Thermo Fisher Scientific

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