Human TGF-beta 1 Recombinant Protein PHG9204 from Thermo Fisher Scientific

Product Specs
ItemHuman TGF-beta 1 Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHG9204
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size5 µg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Super-enhancer profiling reveals ThPOK/ZBTB7B, a CD4+ cell lineage commitment factor, as a master regulator that restricts breast cancer cells to a luminal non-migratory phenotypeCellular and Molecular Life SciencesNovember 13, 2025Camila D. Arcuschin, Kamin Kahrizi, Rosalyn W. Sayaman, Carolina DiBenedetto, Pedro J. Salaberry, Roxana Pirker, Yizhuo Shen, Ons Zakraoui, Cecilia Schwarzer, Alessandro Scapozza, et al.with TGFβ and inhibition of TGBRI Cells were treated with 10 ng/ml of rTGFβ (Life Technologies, cat#: PHG9204) for the indicated times. To assess the activity of TGFβ produced by ThPOK-kd cells, the cells were

(2) A Novel Microfluidic System for 3D Epidermis and Full‐Thickness Skin Growth for Nanoparticle Safety AssessmentAdvanced Healthcare MaterialsNovember 2, 2025Samantha Costa, Ana B. Carneiro, Filipa Lebre, João Meneses, Alar Ainla, Cacilda Moura, Ernesto Alfaro‐Moreno, Ana R. RibeiroMerck), apo‐transferrin (T1147, Merck), hydrocortisone (H0888, Merck), EGF (SRP3027, Merck), TGF‐β (PHG9211, Gibco), and insulin (I9278, Merck). Cellular matrix components included collagen type I from bovine

(3) Basigin links altered skeletal stem cell lineage dynamics with glucocorticoid-induced bone loss and impaired angiogenesisNature communicationsAugust 15, 2025Ambrosi TH, Morales D, Chen K, Hunt EJ, Weldon KC, Maifeld AN, Chavez FIM, Wang Y, Zhao L, Wang L, et al.10% FBS, 100 nM dexamethasone, 1 μM L-ascorbic acid-2-phosphate and 10 ng/mL TGF-β1 (Invitrogen, cat# PHG9204). The micromass was maintained for 21 days with media changes every other day. At the end of differentiation,

(4) Exploring the oncogenic impact of heteroplasmic de novo MT-ND5 truncating mutationsMitochondrial CommunicationsJuly 17, 2025Yuanyuan Wu, Jiangbin Ye, Zhenglong Guwithout 50 μg/ml uridine. When examining the effects of TGFB1 on MCF12A cells, 20 ng/ml of TGFB1 (Gibco, #PHG9204) was supplemented. 4.2. Plasmid construction Polymerase chain reaction (PCR) was performed using Phusion

(5) Yoda1 Inhibits TGFβ-Induced Cardiac Fibroblast Activation via a BRD4-Dependent PathwayCellsJuly 4, 2025Perwez Alam, Sara M. Stiens, Hunter J. Bowles, Hieu Bui, Douglas K. BowlesName,Concentration,Company/Catalog Number TGFβ,10 ng/mL,"Gibco™ (Waltham, MA, USA)/PHG9204" Yoda1,10 µM,"Sigma-Aldrich (St. Louis, MO, USA)/SML1558-5MG" TEA,10 mM,"Sigma-Aldrich (St. Louis,

(6) Structure-Activity Analysis Reveals Perturbed Cilia-Jun N-Terminal Kinase Signaling in MAPKBP1-Associated Kidney DiseaseKidney International ReportsJune 4, 2025Christin Findeisen, Maria Papazian, Linda Pöschla, Anastasia Ertel, Wenjun Jin, Nydia Panitz, Elena Hantmann, Paul Coucke, Firdous Abdulwahab, Lama AlAbdi, et al.with 25 μg/ml anisomycin (AM) (#2222 Cell Signaling, Danvers, MA) or with 10 ng/ml human TGF-beta 1 (# PHG9204, Thermo Fisher Scientific, Waltham, MA) for 1 hour. Cells were treated with 0.1 μM Taxol (Invitrogen,

(7) Gefitinib Facilitates Bone Fracture Healing via Inhibition of the EGFR Pathway and Counteracting SOX9-driven Bone Metabolic ReprogrammingIn vivo (Athens, Greece)May 1, 2025Xu N, Yuan G, Zeng W, Liu X, Wan MA, Xu S, Bi Y, Hu H, Xu Y, Lan S.LRS with 4 μM gefitinib medium containing 10 ng/ml recombinant human transforming growth factor-β1 (PHG9211; Gibco), 50 μM L-ascorbic acid 2-sulphate (Sigma-Aldrich), and 20 μM Rho kinase inhibitor Y-27632 (Sigma-Aldrich)

(8) Microglia aging in the hippocampus advances through intermediate states that drive inflammatory activation and cognitive declineElifeApril 29, 2025Jeremy M. Shea, Saul A. VilledanM) (EMD Millipore, 509265) or human TGFβ1 recombinant protein (10 ng/mL) (Thermo Fisher Scientific, PHG9204) for 24 hours followed by the addition of LPS (200 ng/uL) from Escherichia coli (Sigma-Aldrich, L2018)

(9) YTHDC1 phase separation drives the nuclear export of m6A-modified lncNONMMUT062668.2 through the transport complex SRSF3-ALYREF-XPO5 to aggravate pulmonary fibrosisCell Death DisApril 12, 2025Shengjun Chen, Yujie Wang, Jinjin Zhang, Bo Liu, Weili Liu, Guohong Cao, Rongrong Li, Hongbo Li, Nailiang Zhai, Xiaodong Song, et al.placed in a cell culture incubator at 37 °C with 5% CO2. Cells were treated with 5 ng/mL TGF-β1 (Gibco, PHG9202) for different durations according to experimental requirements. According to experimental requirements,

(10) Genome-wide CRISPR/Cas9 screening identifies key profibrotic regulators of TGF-β1-induced epithelial-mesenchymal transformation and pulmonary fibrosisFrontiers in Molecular BiosciencesFebruary 17, 2025Chunjiang Tan, Juan Wang, Xiangrong Ye, Kaidirina Kasimu, Ye Li, Feng Luo, Hui Yi, Yifeng Luo37°C. On the following day, BEAS-2B cells were treated with human recombinant TGF-β1 (2 μg/mL, rTGF-β1, PHG9204, Gibco, United States) at the concentration of 5 ng/mL, 10 ng/mL, 100 ng/mL, and 1,000 ng/mL for 48

(11) circNR3C2 promotes chondrogenic differentiation and cartilage repair of human adipose‐derived stem cells via the hsa‐miR‐647/SOX9 pathwayAnimal Models and Experimental MedicineFebruary 13, 2025Dabiao Hou, Huajun Wang, Hao Guo, Dongbin Luo, Xiaofei Zheng, Simin Luomedium (Gibco) containing ITS‐1 premix and insulin (I2521, Sigma‐Aldrich), Dex (265005, Merck), TGF‐β1 (PHG9202, ThermoFisher), and FGF (341590, Merck). hADSCs in the 3rd passage (5 × 103/mL) were cultivated in this

(12) SOCS domain targets ECM assembly in lung fibroblasts and experimental lung fibrosisScientific ReportsDecember 30, 2024Carina Magdaleno, Daniel J. Tschumperlin, Narendiran Rajasekaran, Archana Varadarajmyofibroblasts with 5 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1) (Invitrogen, #PHG9204) for 48 h in serum free media. TGF-β1 was reconstituted and stored according to manufacturer’s recommendations. Antibodies

(13) Microglia aging in the hippocampus advances through intermediate states that drive inflammatory activation and cognitive declinebioRxivAugust 20, 2024Jeremy M. Shea, Saul A. Villeda(100nM)(EMD Millipore, 509265) or human TGFβ1 recombinant protein (10ng/mL)(ThermoFisher Scientific, PHG9204) for 24 hours followed by addition of lipopolysaccharides (LPS, 200ng/uL) from E. coli (Sigma-Aldrich,

(14) PAR2 activation on human tubular epithelial cells engages converging signaling pathways to induce an inflammatory and fibrotic milieuFrontiers in PharmacologyJune 28, 2024David A. Vesey, Abishek Iyer, Evan Owen, Danielle Kamato, David W. Johnson, Glenda C. Gobe, David P. Fairlie, David J. Nikolic-PatersonBD Transduction Laboratories, human OptEIA kits; TNF Cat # 555212, GMCSF. Recombinant human TGF-β1 (#PHG9204, Gibco), base growth medium, Gibco Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F12 (Cat #11320)

(15) Plausible role of INPP4A dysregulation in idiopathic pulmonary fibrosisPhysiological reportsMay 1, 2024Arvind M, Pattnaik B, Gheware A, Prakash YS, Srivastava M, Agrawal A, Bhatraju NK.cocktail,Sigma,p8340 5,DAB,, 6,Bicinchoninic acid (BCA),Sigma,B9643‐1 L 7,Human TGF‐β1 recombinant protein,Thermo Fisher,PHG9204 8,Triton X‐100,Sigma,T9284 9,SuperSignalTM West femto maximum sensitivity substrate,Invitrogen,34095 10,SuperSignalTM

(16) Transforming growth factor-induced gene TGFBI is correlated with the prognosis and immune infiltrations of breast cancerWorld Journal of Surgical OncologyJanuary 20, 2024Haiwei Wang, Xinrui Wang, Liangpu Xufor 24 h and then treated with TGFβ 5 ng/ml. Recombinant human TGFβ was purchased from Invitrogen (PHG9202). Microarray analysis The changed genes in MDA-MB-231 cells after TGFβ treatment and the differentially

(17) Optimization of Sirius Red-Based Microplate Assay to Investigate Collagen Production In VitroInt J Mol SciDecember 13, 2023Csenge Szász, Domonkos Pap, Beáta Szebeni, Péter Bokrossy, László Őrfi, Attila J. Szabó, Ádám Vannay, Apor Veres-Székelyconfluence. Cells were then treated with 1 nM recombinant transforming growth factor beta 1 (TGF-β, #PHG9204, Thermo Fisher Scientific) diluted in FBS-free DMEM containing 200 μM ascorbate (Merck) for 48 h unless

(18) Statistical modeling of extracellular vesicle cargo to predict clinical trial outcomes for hypoplastic left heart syndromeiScienceOctober 20, 2023Hoffman JR, Park HJ, Bheri S, Platt MO, Hare JM, Kaushal S, Bettencourt JL, Lai D, Slesnick TC, Mahle WT, et al.Proteins" ,, Matrigel Matrix,Corning,356237 Geltrex,Gibco,A1413302 "Recombinant TGFB1, human",Invitrogen,PHG9204 "Recombinant TNF-alpha, rat",R&D Systems,510-RT "Recombinant basic fibroblast growth factor , human",STEMCELL Technologies

(19) Eos Promotes TH2 Differentiation by Interacting with and Propagating the Activity of STAT5The Journal of ImmunologyAugust 1, 2023Jasmine A. Tuazon, Kaitlin A. Read, Bharath K. Sreekumar, Jack E. Roettger, Michael J. Yaeger, Sanjay Varikuti, Srijana Pokhrel, Devin M. Jones, Robert T. Warren, Michael D. Powell, et al.(clone XMG1.2),0–3,10 μg/ml,BioLegend,505834 TREG,Cytokines,TGF-β,0–3 (or 0–5),2 ng/ml,Invitrogen/Gibco,PHG9204 TREG,Cytokines,IL-2,0–3 (or 0–5),20 ng/ml (50 ng/ml during expansion),Peprotech,200–02 TH2,Neutralizing

(20) Wnt signaling preserves progenitor cell multipotency during adipose tissue developmentNature MetabolismJune 19, 2023Zinger Yang Loureiro, Shannon Joyce, Tiffany DeSouza, Javier Solivan-Rivera, Anand Desai, Pantos Skritakis, Qin Yang, Rachel Ziegler, Denise Zhong, Tammy T. Nguyen, et al.100 nM dexamethasone (cat. no. D1756, Sigma), 10 ng ml−1 Human TGF-β 1 recombinant protein (cat. no. PHG9204, Gibco) and 1 μg ml−1 l-ascorbic acid 2-phosphate (cat. no. A8960, Sigma). The induced cells underwent

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Human TGF-beta 1 Recombinant Protein from Thermo Fisher Scientific

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Human TGF-beta 1 Recombinant Protein from Thermo Fisher Scientific

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