SARS-CoV-2 Spike Protein (RBD) (aa319-541), His Tag Recombinant Protein RP-87678 from Thermo Fisher Scientific

Product Specs
ItemSARS-CoV-2 Spike Protein (RBD) (aa319-541), His Tag Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberRP-87678
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size100 µg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Production of Plant Virus-Derived Hybrid Nanoparticles Decorated with Different NanobodiesACS NanoDecember 17, 2024Enrique Lozano-Sanchez, José-Antonio Daròs, Fernando Merwaissdescribed.32 Briefly, 96 well plates were loaded with 100 ng/well of recombinant RBD (Thermo Scientific, RP-87678) in coating buffer (15 mM Na2CO3, 35 mM NaHCO3, pH 9.8). After washing plates twice with 300 μL of PBS

(2) Oxoammonium salts exert antiviral effects against coronavirus via denaturation of their spike proteinsScientific ReportsOctober 13, 2024Ryosuke Segawa, Yusuke Sasano, Yusuke Hatakawa, Yuto Fujisawa, Shuhei Akutsu, Masanobu Uchimura, Ami Ikura, Kota Matsumoto, Kazuki Sone, Tomoyuki Oe, et al.TEMPO-Oxo and AZADO-Oxo were prepared as previously described24. Histidine-tagged recombinant S-RBD (RP-87678) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Quick CBB PLUS was purchased from FUJIFILM

(3) Generation and Characterization of a Multi-Functional Panel of Monoclonal Antibodies for SARS-CoV-2 Research and TreatmentVirusesDecember 30, 2023Lila D. Patterson, Benjamin D. Dubansky, Brooke H. Dubansky, Shannon Stone, Mukesh Kumar, Charles D. Ricescreening assays. Recombinant RBD (Arg319–Phe541) of the same strain was obtained from Invitrogen (# RP 87678, Waltham, MA, USA) and used as an immunogen without conjugation with KLH and used for screening assays.

(4) Polyaniline/Titania Nanotube-Based Biosensor Strip for Sensitive and Specific Electrochemical Detection of SARS-CoV-2ACS OmegaDecember 5, 2023Al-mahmnur Alam, Timsy Uppal, Md Shariful Islam, Manoranjan Misra, Subhash C Vermawater (the Milli-Q Direct 8 system was used to produce). The SARS-CoV-2 spike protein (catalog no. RP-87678) and anti-SARS-CoV-2 mAb (catalog no. MAB38033) were obtained from Thermo Fisher Scientific. 4.2 Fabrication

(5) Plant virus‐derived nanoparticles decorated with genetically encoded SARS‐CoV‐2 nanobodies display enhanced neutralizing activityPlant Biotechnology JournalNovember 15, 2023Fernando Merwaiss, Enrique Lozano‐Sanchez, João Zulaica, Luciana Rusu, Marta Vazquez‐Vilar, Diego Orzáez, Guillermo Rodrigo, Ron Geller, José‐Antonio DaròsEnzyme‐linked immunosorbent assays Plates (96 wells) were coated with 100 ng/well of recombinant RBD antigen (Thermo Scientific, #RP‐87678) in Coating Buffer (15 mM Na2CO3, 35 mM NaHCO3, pH 9.8). After 2 washes with 300 μL of PBS supplemented

(6) A high CMV-specific T cell response associates with SARS-CoV-2-specific IL-17 T cell productionMedical Microbiology and ImmunologyFebruary 1, 2023Fernanda Tereza Bovi Frozza, Tiago Fazolo, Priscila Oliveira de Souza, Karina Lima, Julia Crispim da Fontoura, Théo Souza Borba, Márcia Polese-Bonatto, Luciane Beatriz Kern, Renato T. Stein, Graham Pawelec, et al.(Kasvi, Brazil) were coated overnight with 1 μg/mL of SARS-CoV-2 Spike Protein (S-RBD; Catalog nr. RP-87678, Invitrogen) or N peptide (Fiocruz, Brazil). Next, blocking buffer was added for 1 h at room temperature.

(7) Uptake of severe acute respiratory syndrome coronavirus 2 spike protein mediated by angiotensin converting enzyme 2 and ganglioside in human cerebrovascular cellsFrontiers in neuroscienceJanuary 1, 2023McQuaid C, Solorzano A, Dickerson I, Deane R.methods Materials SARS-CoV-2 Spike proteins [recombinant SARS-CoV-2 Spike Protein (SP-RBD, Arg319-Phe 541; cat# RP-87678, HEK293 cell expressed and binds ACE2] was obtained from Life Technologies Corporation, Carlsbad, CA,

(8) SARS-CoV-2 infected children form early immune memory responses dominated by nucleocapsid-specific CD8+ T cells and antibodiesFront ImmunolNovember 2, 2022Karina Lima, Julia C. Fontoura, Priscila Oliveira de Souza, Tiago Fazolo, Gabriel Hilario, Renata Zorzetto, Luiz C Rodrigues Junior, Lais D. Coimbra, Alexandre Borin, Karina Bispo-Dos-Santos, et al.software. Enzyme-linked immunosorbent assay Plasma was tested for IgG and IgA antibodies to S-RBD protein (#RP-87678 - Invitrogen, USA), trimeric SARS-CoV-2 spike (S), S protein from variants Delta and Gamma (kindly provided

(9) Multiple early factors anticipate post-acute COVID-19 sequelaeCellMarch 3, 2022Su Y, Yuan D, Chen DG, Ng RH, Wang K, Choi J, Li S, Hong S, Zhang R, Xie J, et al.Solutions,Cat# 210102 "PBS, 1X",Fisher Scientific,Cat# 21-040-CV SARS-CoV-2 RBD Protein,Invitrogen,Cat# RP-87678 SARS-CoV-2 Spike Protein,Invitrogen,Cat# RP-87680 SARS-CoV-2 Nucleocapsid Protein,Invitrogen,Cat#

(10) Albumin Binds COVID-19 Spike 1 Subunit and Predicts In-Hospital Survival of Infected Patients-Possible Alteration by GlucoseJournal of clinical medicineJanuary 25, 2022Zekri-Nechar K, Zamorano-León JJ, Segura-Fragoso A, Alcaide JR, Reche C, Andrés-Castillo A, Martínez-Martínez CH, Giner M, Jiménez-García R, López-de-Andrés A, et al.Sigma-Aldrich, St. Louis, MO, USA). Moreover, experiments using the RBD region of the COVID-19 spike protein (RP-87678, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) were also carried out. After coincubation of

(11) Preclinical Evaluation of Chicken Egg Yolk Antibody (IgY) Anti-RBD Spike SARS-CoV-2—A Candidate for Passive Immunization against COVID-19Vaccines (Basel)January 17, 2022Hendris Wongso et al.recombinant protein of SARS-CoV-2 S-RBD was purchased from Thermo Fisher Scientific (Invitrogen, Cat.no. RP-87678; Waltham, MA, USA). The radioactivity was measured using a dose calibrator (Biodex; Shirley, NY, USA)

(12) Pediatric COVID-19 patients in South Brazil show abundant viral mRNA and strong specific anti-viral responsesNature CommunicationsDecember 1, 2021Tiago Fazolo, Karina Lima, Julia C. Fontoura, Priscila Oliveira de Souza, Gabriel Hilario, Renata Zorzetto, Luiz Rodrigues JÃºnior, Veridiane Maria Pscheidt, Jayme de Castilhos Ferreira Neto, Alisson F. Haubert, et al.above. Enzyme-linked immunosorbent assay (ELISA) Plasma was tested for IgG and IgA antibodies to S-RBD protein (#RP-87678 - Invitrogen, USA) and N protein (kindly provided by Dr. Ricardo Gazinelli - Fiocruz Belo Horizonte,

(13) Spike protein multiorgan tropism suppressed by antibodies targeting SARS-CoV-2Communications biologyNovember 22, 2021Brady M, McQuaid C, Solorzano A, Johnson A, Combs A, Venkatraman C, Rahman A, Leyva H, Kwok WE, Wood RW, et al.systemic inflammation. Methods Materials SARS-CoV-2 SPs (recombinant SARS-CoV-2 SP (S-RBD; cat# RP-87678, HEK293 cell expressed and binds ACE2) and ovalbumin Alexa Fluor-488 conjugate (Cat# 034781; molecular

(14) A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodiesNature communicationsSeptember 17, 2021Chen X, Gentili M, Hacohen N, Regev A.nm. Nanobodies-loaded sensors were dipped in sample buffer containing recombinant RBD (ThermoFisher, RP-87678) for 200 seconds to record association, then dipped in sample buffer for 1200 seconds to record dissociation.

(15) Rapid and accurate agglutination-based testing for SARS-CoV-2 antibodiesCell Rep MethodsJune 21, 2021Esmail S, Knauer MJ, Abdoh H, Voss C, Chin-Yee B, Stogios P, Seitova A, Hutchinson A, Yusifov F, Skarina T, et al.(3,3’,5,5’-Tetramethylbenzidine)",ThermoFisher Scientific,N301 Spike Receptor Binding Domain (RBD) (14μM),ThermoFisher Scientific,RP-87678 Nucleocapsid (full length) (5μM),RayBiotech,230-01104 ACE2 protein,This Study, ,, Software,Software,Software ,, Qupath,https://qupath.github.io/,v0.1.2

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SARS-CoV-2 Spike Protein (RBD) (aa319-541), His Tag Recombinant Protein from Thermo Fisher Scientific

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SARS-CoV-2 Spike Protein (RBD) (aa319-541), His Tag Recombinant Protein from Thermo Fisher Scientific

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