Human IL-4 Recombinant Protein PHC0045 from Thermo Fisher Scientific

Product Specs
ItemHuman IL-4 Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHC0045
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size25 µg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Elevated IgG levels induce an M2‑to‑M1 phenotypic shift in mucosal macrophages and restrict the growth of invasive sphenoid sinus pituitary adenomasMol Med RepApril 14, 2026Xingbo Li, Zhuowei Lei, Sihan Li, Linpeng Xu, Quanji Wang, Qian Jiang, Zihan Wang, Huaqiu Zhang, Yimin Huang, Ting LeiKGaA) and IFN-γ (20 ng/ml; 575302; BioLegend, Inc.), and M2 polarization was induced by 20 ng/ml IL-4 (PHC0045; Thermo Fisher Scientific, Inc.), both for 24 h at 37°C in complete DMEM supplemented with 10% FBS.

(2) Neuroprotective Effects of Desert Milk Exosomes in LPS-Induced Cognitive Decline: Role of Microglial M2 Polarization and AMPK SignalingNutrientsJanuary 19, 2026Yujie Li, Wei Lu, Wentao Qian, Xinyuan Liao, Pengjie Wang, Yi Wang, Wenya Jiao, Menghui Wang, Jingru Zhao, Jinhui Yang, et al.#L2880-100MG, Sigma, USA) stimulation, while the M2 phenotype was induced by 24-h IL-4 (20 ng/mL; cat. #PHC0044, Invitrogen, Carlsbad, CA, USA) treatment. 2.5. Cell Treatment Cell viability was evaluated using

(3) Inhibition of DYRK1B suppresses inflammation in allergic contact dermatitis model and Th1/Th17 immune responseScientific reportsApril 29, 2023Wongchang T, Pluangnooch P, Hongeng S, Wongkajornsilp A, Thumkeo D, Soontrapa K.(16–7048-85, eBioscience), and 50 U/mL IL-2 (589104, BioLegend) for Th1 differentiation; with 30 ng/mL IL-4 (PHC0044, Gibco), 5 µg/mL anti-IFN-γ Ab (16-7318-81, eBioscience), and 50 U/mL IL-2 (589,104, BioLegend) for

(4) Epstein-Barr Virus-Encoded Circular RNA CircBART2.2 Promotes Immune Escape of Nasopharyngeal Carcinoma by Regulating PD-L1Cancer researchOctober 1, 2021Ge J, Wang J, Xiong F, Jiang X, Zhu K, Wang Y, Mo Y, Gong Z, Zhang S, He Y, et al.and added to 10% FBS-1640 medium containing 50 ng/m LGM-CSF (PHC2013, Invitrogen) and 20 ng/mL IL4 (PHC0044, Invitrogen) for 5 days. To promote the maturation of dendritic cells, 25 ng/mL IFNγ (Invitrogen) was

(5) Macrophage polarization-associated lnc-Ma301 interacts with caprin-1 to inhibit hepatocellular carcinoma metastasis through the Akt/Erk1 pathwayCancer cell internationalAugust 10, 2021Luo HL, Luo T, Liu JJ, Wu FX, Bai T, Ou C, Chen J, Li LQ, Zhong JH.ng/mL, P1585, Sigma-Aldrich, USA) for 24 h, followed by the addition of interleukin (IL)-4 (20 ng/mL, PHC0044, Thermo Fisher Scientific, USA) and IL-13 (20 ng/mL, PHC0135, Thermo Fisher Scientific, USA) for 12

(6) Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteinsCell reportsMay 11, 2021Hiatt J, Cavero DA, McGregor MJ, Zheng W, Budzik JM, Roth TL, Haas KM, Wu D, Rathore U, Meyer-Franke A, et al.30–002-CI), 1mM Sodium Pyruvate, 50ng/mL GM-CSF (Life Technologies PHC2015), 50ng/mL IL-4 (Life Technologies PHC0045); for macrophage differentiation: 20% Human AB Serum, Penicillin-Streptomycin, 1mM Sodium Pyruvate.

(7) Baby's First Macrophage: Temporal Regulation of Hofbauer Cell Phenotype Influences Ligand-Mediated Innate Immune Responses across GestationJournal of immunology (Baltimore, Md. : 1950)May 1, 2020Swieboda D, Johnson EL, Beaver J, Haddad L, Enninga EAL, Hathcock M, Cordes S, Jean V, Lane I, Skountzou I, et al.50ng/mL LPS (00–4976) (Thermo); 100IU/mL IFN-α A/D (PHC4044), 20ng/mL IFN-γ (PHC4031), 20ng/mL IL-4 (PHC0044), 20ng/mL IL-13 (PHC0134) (Life Technologies); 100ng/mL IL-29 (ab50032), 20ng/mL IL-10 (ab9613) (Abcam);

(8) Nivolumab Enhances In Vitro Effector Functions of PD-1(+) T-Lymphocytes and Leishmania-Infected Human Myeloid Cells in a Host Cell-Dependent MannerFrontiers in immunologyJanuary 1, 2017Filippis C, Arens K, Noubissi Nzeteu GA, Reichmann G, Waibler Z, Crauwels P, van Zandbergen G.Systems), or human monocyte-derived dendritic cells (hMDDC) (5 ng/mL GM-CSF; 10 ng/mL human IL-4, Gibco®, PHC0045) for a period of 5 days at 37°C, 5% CO2 as described (27). CD14− cells or peripheral blood lymphocytes

(9) The tumor-associated antigen RHAMM (HMMR/CD168) is expressed by monocyte-derived dendritic cells and presented to T cellsOncotargetNovember 8, 2016Willemen Y, Van den Bergh JM, Bonte SM, Anguille S, Heirman C, Stein BM, Goossens H, Kerre T, Thielemans K, Peeters M, et al.granulocyte-macrophage colony-stimulating factor (cat# CTK-221, Gentaur) and 20 ng/mL interleukin (IL)-4 (cat# PHC0041, Thermo Fisher Scientific). After 5 days, 20 ng/mL tumor necrosis factor-α (cat# CTK-223, Gentaur) and

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Human IL-4 Recombinant Protein from Thermo Fisher Scientific

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Human IL-4 Recombinant Protein from Thermo Fisher Scientific

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