Human TNF-alpha Recombinant Protein PHC3016 from Thermo Fisher Scientific

Product Specs
ItemHuman TNF-alpha Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHC3016
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size50 µg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Discovery of kidney disease targets using multimodal human podocyte injury modelsStem Cell ReportsApril 14, 2026Amanda D. Barreto, Bowen Jiang, Morgan A. Burt, Nikolaos Dimitrakakis, Samira Musahdiabetic medium), prepared by adding IL-6 (PeproTech, 200-06, NJ, USA) and TNFα (Thermo Fisher Scientific, PHC3015, MA, USA) to HG medium; (3) ADR (0.5 μg/mL, LC Laboratories, D4000, MA, USA); (4) Cyto D (0.5 μg/mL,

(2) Establishment and characterization of a new immortalized human adenomyosis epithelial-like cell line, tAEC21†Biology of ReproductionApril 13, 2026Yuliya Klymenko, Jessica L Kersey, Hunter D Quigley, Shannon M Hawkinsin serum-free media overnight. Cells were treated with 15 ng/ml tumor necrosis factor-alpha (TNF-α; #PHC3011, Gibco) or vehicle (DI water) for 24 h. Steroid hormonal treatment For two-dimensional (2D) experiments,

(3) A deep learning approach to assess transendothelial cell trafficking performanceSci RepApril 3, 2026Thomas Michael Schumacher, Elisabeth Marie Gottloeber, Eric Koziel, Mehmet Sacma, Kaya Eichhorn, Luana Raiber, Johann Gout, Jessica Lindenmayer, Elodie Roger, Michael Karl Melzer, et al.replaced with growth medium containing TNF-α (Gibco, ThermoFisher Scientific, Grand Island, NY, USA, #PHC3011) at 10 ng/ml following incubation for 24 h at 37 °C with 5 % CO2 prior to flow-assay. The TNF-α containing

(4) High‐Content SRS Imaging Unveils Altered Cholesterol Metabolism in Ovarian Cancers Under CAR‐T TreatmentAdvanced ScienceFebruary 15, 2026Chinmayee V. Prabhu Dessai, Zhuoying Huang, Haonan Lin, Guangrui Ding, Hongjian He, Menna Siddiqui, Meng Zhang, Wilson Wong, Ji‐Xin Chengsupplemented with 100 IU/mL IL‐2 (NCI BRB Preclinical Repository), 10 ng/mL TNF‐𝛼 (Thermo Fisher Scientific, PHC3011), 5 ng/mL IFN‐𝛾 (Thermo Fisher Scientific, PHC4033) respectively for 24 h. The cells were then fixed

(5) Comparative toxicity of menthol- and tobacco-flavored electronic cigarette constituents inducing inflammation, epithelial barrier dysfunction, and nicotinic acetylcholine receptor modulation in the absence of nicotineToxicology ReportsFebruary 14, 2026Vidhi S. Pandya, Arni Bhatnagar, Kirby J. Beck, Thivanka Muthumalage0.1 %. Hydrogen peroxide (100 µM) (Fisher Scientific, Catalog: H325–500) and TNFα (10 ng/µL) (Gibco, PHC3015) were used as positive controls for oxidative stress-driven injury and inflammation, respectively. 2.3

(6) Beyond antipsychotic efficacy and toward an individualized therapeutic strategy: analysis of the systemic, metabolic, and inflammatory effects of olanzapine, haloperidol, and their combination in schizophrenic patientsFrontiers in PharmacologyFebruary 3, 2026Nayef Samah Alharbi, Noha Alaa Hamdy, Esam M. Aboubakr, Mansour Alharbi, Mostafa A. Ali, Ghaleb Alharbi, Ahmed Ibrahim ElMallah1:300), eBioscience, Thermosientific, USA, and Human TNF-α Recombinant Protein (MW: 17.5 kDa), Catalog #PHC3011 (dilution 1:500), (Gibco, Thermosientific, USA). The membrane was examined at 340 nm using the Analytik

(7) CRISPR screens in the context of immune selection identify CHD1 and MAP3K7 as mediators of cancer immunotherapy resistanceCell Reports MedicineJanuary 20, 2026Alex Watterson, Gabriele Picco, Vivien Veninga, Youhani Samarakoon, Chiara M. Cattaneo, Sara F. Vieira, Emre Karakoc, Shriram Bhosle, Thomas W. Battaglia, Sarah Consonni, et al.proteins" ,, IFN-γ,Thermo Fisher,Cat.# 300-02-100 μg IFN-β,STEMCELL Technologies,Cat.# 78113.1 TNF-α,Thermo Fisher,Cat.# PHC3011 IL-6,Thermo Fisher,Cat.# PHC0064 Basement membrane extract (BME),R&D Systems,Cat.# 3433-010-R1 Fixable

(8) Proteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesisbioRxivJanuary 17, 2026Drew W. Grant, Shengjiang Tan, Dylan E. Ramage, Mamie Z. Li, Ying Di, Iva A. Tchasovnikarova, Michael P. Weekes, Stephen J. Elledge, Alan J. Warren, Richard T. Timmsfinal concentration of 250 nM. Recombinant human TNF-α was purchased from ThermoFisher Scientific (#PHC3015) and used at a final concentration of 10 ng/ml; recombinant human interferon-ɣ was purchased from Cambridge

(9) Inflammatory Co-Regulation of Voltage-Gated Sodium Channels and Na,K-ATPase in Metastatic Breast CancerInternational Journal of Molecular SciencesDecember 31, 2025Steven D. Scahill, Kelly Jean Sherman, Dennis Paul(pen/strep) (Gibco, 15070-063) at 5% CO2 and 37 °C. Cells were treated with 3.76 or 14.14 ng/mL TNFα (Gibco, PHC3016), 100 nM protoxin-1 (Alamone Labs, Jerusalem, Israel, STP-400), and 10 µM or 100 nM ouabain (Sigma-Aldrich,

(10) Decoding necrosome assembly: harmonizing signal amplification and attenuation through optimal RIP3 stoichiometryNature CommunicationsDecember 23, 2025Xiang Li, Yating Cao, Fei Xu, Yiting Zhang, Yue Kong, Chengjie Lan, Rongfeng Zhu, Cheng Lin, Chuan-Qi Zhong, Zhilong Liu, et al.Qinxi Li at Xiamen University). All plasmids were verified by DNA sequencing. Recombinant human TNF-α (PHC3011) was from Thermo Fisher Scientific and used at 0.1–10 ng/mL. The following compounds were used: Smac

(11) SMAC mimetics sensitize HIV-infected cells to oncolytic virus-mediated deathFront ImmunolDecember 18, 2025Bengisu Molyer, Yasmeen Ameeriar, Jonathan B. Angelpurchased from BioLegend (cat. no. 574802; San Diego, CA, USA). Human TNFα recombinant protein (cat, no. PHC3015) was purchased from Thermo Fisher Scientific. The bivalent SMAC mimetic AEG-40730 (cat. no. 5330) was

(12) Vessel-on-a-Chip to Study Vascular Endothelial InflammationCurr ProtocDecember 18, 2025Svitlana M. Palii, Anastasiia Voytovych, Nadiya Muzyka, Nuria Chantada, Pablo J. Sáez, Ezequiel Álvarez, Oksana Shevchuk3). Additional materials (also see Basic Protocols 1 and 2) Recombinant Human TNF (Gibco, cat. no. PHC3016)PBS (Merck, P3813)4% (w/v) paraformaldehyde (AlfaAesar, cat. no. J67899)0.2% (v/v) Triton X‐100 (Sigma,

(13) Single‐Cell Metabolic Imaging Reveals Glycogen‐Driven Adaptations in Endothelial CellsAdvanced ScienceNovember 26, 2025Rahuljeet S. Chadha, Benjamin Yang, Dongqiang Yuan, Edmund D. Kapelczak, Philip A. Kocheril, Alonso Tapia, Shivansh Mahajan, Adrian Colazo, Naseeb K. Malhi, Joseph A. Ambarian, et al.6,6‐d2‐glucose (Cambridge Isotope Laboratories, Cat. #DLM‐349), along with 5 ng mL−1 TNF‐α (Gibco, Cat. #PHC3015). The NM condition included addition of 25 mM mannitol (Thermo Fisher, Cat. #125340025) as an osmolarity

(14) Impact of preoperative bowel preparation methods on anastomotic leakage and intestinal motility recovery in laparoscope-assisted heart-shaped anastomosisFront SurgOctober 3, 2025Pei Zhang, Decheng Wei, Jian Bian, Shijin QiThermo Fisher Scientific Inc., USA), IL-10 (EPX01A-10215-901, Thermo Fisher Scientific Inc., USA), and TNF-α (PHC3016, Thermo Fisher Scientific Inc., USA). 2.4.4 Microbiological biomarkers Specimens for microbiological

(15) Comparing the Metabolic, Systemic, and Neuropsychiatric Impacts of Olanzapine and Clozapine in Patients with SchizophreniaPharmaceuticals (Basel)September 1, 2025Nayef Samah Alharbi, Noha Alaa Hamdy, Esam M. Aboubakr, Mansour Alharbi, Mostafa A. Ali, Ghaleb Alharbi, Ahmed Ibrahim ElMallaheBioscience, Thermo Scientific, Waltham, MA, USA), and Human TNF-α Recombinant Protein (MW: 17.5 kDa; catalog #PHC3011; dilution 1:500; Gibco, Thermo Scientific, Waltham, MA, USA) were used to determine the IL-6, IL-1β,

(16) Development of a High-throughput Morphological Assay for Evaluating Mesenchymal Stromal Cell-derived Extracellular Vesicle Modulation of Brain Pericyte Secretory PhenotypeStem Cell Reviews and ReportsAugust 12, 2025Courtney E. Campagna, Andrew M. Larey, Kanupriya R. Daga, Morgan Roos, Sneha Ghosh, Neil Grimsey, Jin Han, Ross A. Markleinpericyte medium only (‘unstimulated’) or 50 µL of pericyte medium containing 50 ng/mL TNF-α (Gibco, PHC3015) (‘stimulated’) were added to appropriate wells and pericytes incubated for an additional 24 h. For

(17) Dynamic decoding of VEGF signaling and coordinated control of multiple phenotypes by the Src-TEM4-YAP pathwayCell SystemsJuly 16, 2025Sung Hoon Lee, Tae-Yun Kang, Xingbo Shang, Andre LevchenkoHuman VEGF-165 Recombinant Protein,Thermo Fisher,Cat# PHC9364 Human TNF-α recombinant protein,Thermo Fisher,Cat# PHC3015 Oleoyl-L-α-lysophosphatidic acid sodium salt,Millipore Sigma,Cat# L7260 Dasatinib,Selleckchem,Cat#

(18) Molecular insights into the relationship between sustained CRP elevation and endothelial dysfunction in axial spondyloarthritisRMD OpenJuly 5, 2025Laura Cuesta-López, Iván Arias-de la Rosa, Jesús Eduardo Martín-Salazar, Antonio Manuel Barranco, Lourdes Ladehesa-Pineda, Miriam Ruiz-Ponce, María Ángeles Puche Larrubia, Carlos Pérez-Sánchez, Pedro Seguí, Rafaela Ortega, et al.ng/mL and 500 ng/mL) (LSBio, LS-G132833) in combination with 50 ng/mL TNF- a (Thermo Fisher Scientific, PHC3015L).22 In the second set of experiments, HUVECs were treated for 24 hours with 10% serum from healthy donors

(19) DR5 Disulfide Bonding Functions as a Sensor and Effector of Protein Folding StressMolecular Cancer ResearchJuly 2, 2025Mary E. Law, Zaafir M. Dulloo, Samantha R. Eggleston, Gregory P. Takacs, Grace M. Alexandrow, Young il Lee, Mengxiong Wang, Brian Hardy, Hanyu Su, Bianca Forsyth, et al.Bafilomycin A1, Gefitinib, ISRIB, MK2206, and Q-VD-OPH: Cayman Chemical (Ann Arbor, MI, USA); TNF, (PHC3016); N-ethylmaleimide (NEM): Thermo Fisher Scientific (Grand Island, NY, USA); eEF2K inhibitor (A-484954),

(20) Orientia tsutsugamushi Modulates RIPK3 Cellular Levels but Does Not Inhibit NecroptosisPathogensMay 14, 2025Thomas E. Siff, Paige E. Allen, David L. Armistead, Jason R. Hunt, Steven J. Rolland, Hervé Agaisse, Jason A. Carlyonwith fresh media containing 1 mM BV6, 20 mM zVAD-fmk, and 10 ng/mL tumor necrosis factor (TNF; Gibco PHC3015) (TBZ) or vehicle (DMSO and filter-sterilized 0.1% BSA in deionized water). Cultures were incubated

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Human TNF-alpha Recombinant Protein from Thermo Fisher Scientific

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Human TNF-alpha Recombinant Protein from Thermo Fisher Scientific

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