Human IL-21 Recombinant Protein PHC0215 from Thermo Fisher Scientific

Product Specs
ItemHuman IL-21 Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberPHC0215
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size25 µg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Dual Phospho-CyTOF Workflows for Comparative JAK/STAT Signaling Analysis in Human Cryopreserved PBMCs and Whole BloodBIO-PROTOCOLNovember 20, 2025Ilyssa E. Ramos, Brynja Matthiasardottir, Teresa S. Hawley, Kyu Lee Han, Michal Toborek, Iyadh Douagi, Georgette N. Jones, James M. Cherrycell culture (Sigma-Aldrich, catalog number: L7770-1MG) 4. IL-21 (Fisher Scientific, catalog number: PHC0214) 5. RPMI 1640 medium, GlutaMAXTM supplement (Thermo Fisher Scientific, catalog number: 61870036) 6.

(2) A human milk oligosaccharide alters the microbiome, circulating hormones, and metabolites in a randomized controlled trial of older adultsCell Reports MedicineAugust 19, 2025Matthew M. Carter, Diane Demis, Dalia Perelman, Michelle St. Onge, Christina Petlura, Kristen Cunanan, Kavita Mathi, Holden T. Maecker, Jo May Chow, Jennifer L. Robinson, et al.,BD Biosciences,Cat#550071 IL-7,BD Biosciences,Cat#554608 IL-10,BD Biosciences,Cat#554611 IL-21,Fisher Scientific,Cat#PHC0214 ,, Biological samples,Biological samples,Biological samples ,, Human stool samples,This study, Human

(3) Protective antibodies target cryptic epitope unmasked by cleavage of malaria sporozoite proteinScienceJanuary 3, 2025Cherrelle Dacon, Re’em Moskovitz, Kristian Swearingen, Lais Da Silva Da Silva Pereira, Yevel Flores-Garcia, Maya Aleshnick, Sachie Kanatani, Barbara Flynn, Alvaro Molina-Cruz, Kurt Wollenberg, et al.in IMDM (Gibco, 31980-030) supplemented with 10% HI-FBS (Gibco, 10438-026), 100 ng/mL IL21 (Gibco, PHC0211), 0.5 μg/mL R848 (Invivogen, tlrl-r848) and 1X Mycozap (Lonza, VZA-2021). Cultures were maintained at

(4) Twin study dissects CXCR3+ memory B cells as non-heritable feature in multiple sclerosisMedApril 12, 2024Florian Ingelfinger, Kirsten L. Kuiper, Can Ulutekin, Lukas Rindlisbacher, Sarah Mundt, Lisa Ann Gerdes, Joost Smolders, Marvin M. van Luijn, Burkhard Becherproteins","Chemicals, peptides, and recombinant proteins" ,, Human IL-21 Recombinant Protein,Thermo Fisher,PHC0215 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Human CXCL10/IP-10

(5) Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolutionCell ReportsMarch 26, 2024Michelle K. Tenggara, Seo-Ho Oh, Catherine Yang, Hardik K. Nariya, Amanda M. Metz, Amit A. Upadhyay, Dedeepya R. Gudipati, Lizheng Guo, Emily G. McGhee, Kiran Gill, et al.hydrochloride,Sigma Aldrich,Cat# D9885–10G Human IL-2,This paper,N/A Human IL-21,ThermoFisher Scientific,Cat# PHC0213 Human IL-4,Miltenyi Biotec,Cat# 130-093-922 Human BAFF,GenScript,Cat# Z02976–1 HIV-1 BG505.DS.SOSIP.S613A

(6) Rare, convergent antibodies targeting the stem helix broadly neutralize diverse betacoronavirusesCell host & microbeJanuary 11, 2023Dacon C, Peng L, Lin TH, Tucker C, Lee CD, Cong Y, Wang L, Purser L, Cooper AJR, Williams JK, et al.in IMDM (Gibco, 31980-030) supplemented with 10% HI-FBS (Gibco, 10438-026), 100 ng/mL IL21 (Gibco, PHC0211), 0.5 μg/mL R848 (Invivogen, tlrl-r848) and 1× Mycozap (Lonza, VZA-2021). Sf9 and High Five cells were

(7) Broadly neutralizing antibodies target the coronavirus fusion peptidebioRxivSeptember 16, 2022Cherrelle Dacon et al.31980-030) supplemented with 10% heat-inactivated (HI)-FBS (Gibco, 10438-026), 100 ng/mL IL21 (Gibco, PHC0211), 0.5 μg/mL R848 (Invivogen, tlrl-r848) and 1× Mycozap (Lonza, VZA-2021). The cell suspension was seeded

(8) Potent neutralization by monoclonal human IgM against SARS-CoV-2 is impaired by class switchEMBO reportsJuly 5, 2022Callegari I, Schneider M, Berloffa G, Mühlethaler T, Holdermann S, Galli E, Roloff T, Boss R, Infanti L, Khanna N, et al.plate wells at an average density of 0.9 cells/well, in RPMI with 40% FCS, 0.05 ng/µl IL‐21 (Gibco, PHC0215), together with 50,000 irradiated TE CD40L cells/well. After 9 days of culture, 15 µl of supernatant

(9) Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole BloodMethods in molecular biology (Clifton, N.J.)January 1, 2022Sigal N, Maecker HT.,BD Biosciences cat. # 554611,50 ng/ml IL17a,Peprotech Catalog # 200-17,50 ng/ml IL21,Life Technologies cat. # PHC0214,50 ng/ml LPS,Sigma cat. # L7770,1 ug/ml PMA,Sigma cat. # P8139,10 ng/ml Ionomycin,Calbiochem cat.

(10) Functional human IgA targets a conserved site on malaria sporozoitesScience translational medicineJune 23, 2021Tan J, Cho H, Pholcharee T, Pereira LS, Doumbo S, Doumtabe D, Flynn BJ, Schön A, Kanatani S, Aylor SO, et al.containing 100 U/mL interleukin (IL)-2 (Roche 11147528001) and 50 ng/mL IL-21 (Thermo Fisher Scientific, PHC0211). Cells were cultured for 2 weeks following which supernatants were harvested and screened by high-throughput

(11) Extremely potent human monoclonal antibodies from COVID-19 convalescent patientsCellApril 1, 2021Andreano E, Nicastri E, Paciello I, Pileri P, Manganaro N, Piccini G, Manenti A, Pantano E, Kabanova A, Troisi M, et al.Recombinant Human Protein,Thermo Fisher,Cat#PHC0023 IL-21 Recombinant Human Protein,Thermo Fisher,Cat#PHC0211 Strep-Tactin DY488,IBA lifesciences,Cat#2-1562-050 Slide-A-Lyzer™ Dialysis Cassettes,Thermo Fisher,Cat#66003 HiTrap Protein G HP

(12) Mapping Neutralizing Antibody Epitope Specificities to an HIV Env Trimer in Immunized and in Infected Rhesus MacaquesCell reportsSeptember 8, 2020Zhao F, Joyce C, Burns A, Nogal B, Cottrell CA, Ramos A, Biddle T, Pauthner M, Nedellec R, Qureshi H, et al.Healthcare,Cat#17-1279-03 Recombinant human IL-2,Roche,Cat#11147528001 Recombinant human IL-21,Invitrogen,Cat#PHC0215 Recombinant human IL-4,Miltenyi,Cat#130-093-922 Alkaline phosphatase-conjugated streptavidin,Jackson ImmunoResearch

(13) Isolating Human Monoclonal Antibodies Against Adeno-Associated Virus From Donors With Pre-existing ImmunityFrontiers in ImmunologyJuly 7, 2020April R. Giles, Roberto Calcedo, Anna P. Tretiakova, James M. Wilsonby adding 17,500 U IL-2 (Cat. 11147528001, Roche Diagnostics, Indianapolis, IN), 87.5 μg IL-21 (Cat. PHC0215, Life Technologies, Carlsbad, CA), and 1.75e8 irradiated 3T3-msCD40L cells to 1,680 mL IMDM with GlutaMAX

(14) Broad and Potent Neutralizing Antibodies Recognize the Silent Face of the HIV EnvelopeImmunityJune 18, 2019Schoofs T, Barnes CO, Suh-Toma N, Golijanin J, Schommers P, Gruell H, West AP Jr, Bach F, Lee YE, Nogueira L, et al.12338001 Interleukin 2 (IL-2),Roche,Cat# 11147528001 Interleukin 21 (IL-21),Life Technologies,Cat# PHC0211 Streptavidin-PE,BioLegend,Cat# 405203 BG505-SOSIP.664.Avi,"John P. Moore, Weill Cornell Medical College;

(15) Identification of neoantigen-specific T cells and their targets: implications for immunotherapy of head and neck squamous cell carcinomaOncoImmunologyApril 3, 2019Lili Ren et al.cells/expanded TILs in CellGro DC/5% ABS with 30 ng/mL of IL-21 (Thermo Fisher Scientific, Catalog number PHC0214) (each well in a 48-well plate contained 1.0 × 105 peptide-pulsed DCs and 5 × 105 CD8+ T cells). On

(16) BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-β receptor type I ALK5PloS oneJanuary 1, 2017Bollum LK, Huse K, Oksvold MP, Bai B, Hilden VI, Forfang L, Yoon SO, Wälchli S, Smeland EB, Myklebust JH.Ligands (ALX-850-064) were acquired from Alexis Biochemicals, Enzo Life Sciences (NY, USA). IL-21 (PHC0214) was purchased from Invitrogen (CA, USA) and from eBioscience. BMP-2, BMP-4, BMP-6 and Activin A were

(17) Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass CytometryBio-protocolJune 5, 2015Fernandez R, Maecker H.554608)IL10 (BD Biosciences, catalog number: 554611)IL21 (Life Technologies, Gibco®, catalog number: PHC0214)IL2 (BD Biosciences, catalog number: 554603)CD3 (BD Biosciences, catalog number: 555329)CD28 (BD Biosciences,

(18) Cytokine-stimulated Phosphoflow of PBMC Using CyTOF Mass CytometryBio-protocolJune 5, 2015Fernandez R, Maecker H.IL10 (BD Biosciences, catalog number: 554611) IL21 (Life Technologies, Gibco®, catalog number: PHC0214) IL2 (BD Biosciences, catalog number: 554603) CD3 (BD Biosciences, catalog number: 555329) CD28

(19) Isolation of human monoclonal antibodies from peripheral blood B cellsNature ProtocolsOctober 1, 2013Jinghe Huang et al.10,000 U ml−1 (Roche, cat. no. 11147528001) Recombinant human IL-21, 25 μg (Invitrogen, cat. no. PHC0215) PBS, pH 7.4, 1× (Lonza, cat. no. 17-516F) Guava ViaCount (EMD Millipore, cat. no. 4000-0041)

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Human IL-21 Recombinant Protein from Thermo Fisher Scientific

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Human IL-21 Recombinant Protein from Thermo Fisher Scientific

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