Human TNF-alpha Recombinant Protein RP-75738 from Thermo Fisher Scientific

Product Specs
ItemHuman TNF-alpha Recombinant Protein
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberRP-75738
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Size1 mg
ApplicationsCELL CULTURE

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) The soluble (pro)renin receptor promotes a preeclampsia-like phenotype both in vitro and in vivoHypertension research : official journal of the Japanese Society of HypertensionJune 1, 2024Schofield LG, Delforce SJ, Pryor JC, Endacott SK, Lumbers ER, Marshall SA, Pringle KG.nmol/L s(P)RR-Flag protein (CSIRO, Clayton Australia), or 10 ng/mL TNF-α (positive control; Gibco Cat: RTNFAI, Lot:2285310) [23–25]. HUtMECs were treated for 24 h before cells and supernatant were collected, snap

(2) Combination of bazedoxifene with chemotherapy and SMAC-mimetics for the treatment of colorectal cancerCell Death & DiseaseApril 10, 2024Rhynelle S. Dmello, Michelle Palmieri, Pathum S. Thilakasiri, Larissa Doughty, Tracy L. Nero, Ashleigh R. Poh, Sarah Q. To, Erinna F. Lee, W. Douglas Fairlie, Lisa Mielke, et al.BMS341), human recombinant IL-11 (Thermo Fisher; PHC0115) and human recombinant TNF-α (Thermo Fisher; RP75738). Flow cytometry For Annexin V TO-PRO-3-iodide staining, adherent cells were treated with combinations

(3) Luteolin prevents TNF-α-induced NF-κB activation and ROS production in cultured human placental explants and endothelial cellsPlacentaJanuary 1, 2024Adrian C. Eddy, Augustine Rajakumar, Frank T. Spradley, Joey P. Granger, Sarosh Ranadimethyl sulfoxide (DMSO) control for 6 hours before stimulation with TNF-α (50ng/mL) (Pierce Biotechnology; Cat# RTNFAI; Rockford, IL). Luteolin concentrations were chosen based on our previous studies [19]. After 24 h,

(4) Remifentanil does not affect human microglial immune activation in response to pro-inflammatory cytokinesEXCLI JFebruary 24, 2023Cinzia Dello Russo, Natalia Cappoli, Elisabetta Tabolacci, Liliana Sollazzi, Pierluigi Navarra, Paola AcetoScientific, Waltham, MA, USA). The recombinant pro-inflammatory cytokines, namely the human TNFα (cat. n. RTNFAI) and the human IL-1β (cat. n. RIL1BI) were from Invitrogen (Thermo Fisher Scientific). The human interferon-γ

(5) Specific S100 Proteins Bind Tumor Necrosis Factor and Inhibit Its ActivityInternational Journal of Molecular SciencesDecember 15, 2022Alexey S. Kazakov, Marina Y. Zemskova, Gleb K. Rystsov, Alisa A. Vologzhannikova, Evgenia I. Deryusheva, Victoria A. Rastrygina, Andrey S. Sokolov, Maria E. Permyakova, Ekaterina A. Litus, Vladimir N. Uversky, et al.human sTNF with an N-terminal hexahistidine tag from ThermoFisher Sci. (Waltham, MA, USA) (catalog # RP-75738) was used for MTT assay. Recombinant human TNF-β (UniProt entry P01374) produced in E. coli was from

(6) Calmodulin Binding Activates Chromobacterium CopC Effector to ADP-Riboxanate Host Apoptotic CaspasesmBioJune 28, 2022Yaxin Liu, Huan Zeng, Yanjie Hou, Zilin Li, Lin Li, Xiaocui Song, Jingjin Ding, Feng Shao, Yue Xuprovided by Jianhua Sui (National Institute of Biological Sciences, Beijing). Recombinant human TNF-α (RTNFAI), Fluo-4 AM (F14201), and BAPTA-AM (B6769) were from Invitrogen. Cycloheximide (HY-12320), recombinant

(7) An experimental model of the epithelial to mesenchymal transition and pro-fibrogenesis in urothelial cells related to bladder pain syndrome/interstitial cystitisTranslational andrology and urologyNovember 1, 2021Jin XW, Wang QZ, Zhao Y, Liu BK, Zhang X, Wang XJ, Lu GL, Pan JW, Shao Y.serum-free F12K medium containing 2, 10, or 50 ng/mL of tumor necrosis factor (TNF)-α (Invitrogen, #RTNFAI, USA). SV-HUC-1 cells cultured with only serum-free F12K served as a control. The medium was refreshed

(8) The mTOR kinase inhibitor rapamycin enhances the expression and release of pro-inflammatory cytokine interleukin 6 modulating the activation of human microglial cellsEXCLI journalJanuary 1, 2019Cappoli N, Mezzogori D, Tabolacci E, Coletta I, Navarra P, Pani G, Dello Russo C.The recombinant pro-inflammatory cytokines, namely the human tumor necrosis factor α (TNFα, cat. n. RTNFAI) and the human interleukin-1β (IL-1β, cat. n. RIL1BI) were from Invitrogen (Thermo Fisher Scientific)

(9) Regulation of Linear Ubiquitin Chain Assembly Complex by Caspase-Mediated Cleavage of RNF31Molecular and cellular biologyDecember 15, 2016Joo D, Tang Y, Blonska M, Jin J, Zhao X, Lin X.h, cells were lysed for further experiments. Reagents and plasmid. Human TNF-␣ recombinant protein (RTNFAI; Thermo Scientific), a de novo protein synthesis inhibitor (cycloheximide [CHX]; Enzo), a proteasome

(10) ROCK inhibitor reduces Myc-induced apoptosis and mediates immortalization of human keratinocytesOncotargetOctober 11, 2016Dakic A, DiVito K, Fang S, Suprynowicz F, Gaur A, Li X, Palechor-Ceron N, Simic V, Choudhury S, Yu S, et al.05–201, clone: CH11), TNF-α (10 ng/ml) (Thermo scientific-Pierce; Rockford Illinois, USA; Cat # EN-RTNFAI), Etoposide (50 μM) (MP Biomedicals; Santa Ana, California, USA; Cat # ICN19391825), or Camptothecin

(11) Downregulation of vimentin in macrophages infected with live Mycobacterium tuberculosis is mediated by Reactive Oxygen SpeciesScientific reportsFebruary 15, 2016Mahesh PP, Retnakumar RJ, Mundayoor S.(G1918, Sigma) −500 nM, DPI (D2926, Sigma) −10 μM, LPS (L4391, Sigma) −1 μg/ml and recombinant TNFα (RTNFAI, Thermo) −10 ng/ml. 2D gel electrophoresis After 24 h of infection, total protein of infected and

(12) Time-Resolved Fluorometric Method for One-Step Immunoassays Using Plasmonic NanostructuresThe journal of physical chemistry. C, Nanomaterials and interfacesApril 29, 2010Szmacinski H, Murtaza Z, Lakowicz JR.(Rockwood, IL). The TNF-α assay consisted of components of the ELISA kit including recombinant human TNF-α (RTNFAI), capture monoclonal antibody (MTNFAI, binding affinity of 0.14 nM) and secondary detection monoclonal

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Human TNF-alpha Recombinant Protein from Thermo Fisher Scientific

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Human TNF-alpha Recombinant Protein from Thermo Fisher Scientific

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