Description
Recombinant full-length human PKCzeta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_002744.
PKCzeta is an atypical isoform of the PKC family. PKCzeta is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Overexpression of PKCzeta and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation (1). Overexpression of PKCzeta also potentiates phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. PKCzeta is an upstream modulator of p70S6K, an important regulator of cell proliferation (2)