Description
Short Description: Recombinant Mononucleosomes H3K14ac (EPL)-biotinylated consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NP_003503.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3.2 that includes amino acids 1-136 (end)(accession number NP_066403.2) with acetylation at lysine 14, and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). All of these histones were expressed in E Coli. The molecular weight of histone octamer is ~108 kDa. The recombinant protein is >=95% by SDS-PAGE gel. H3K14ac (Histone H3 acetyl Lys14) protein is generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E Coli and purified using FPLC. The purified protein is subsequently ligated to an N-terminal histone tail peptide containing acetyl lysine 14 via a native peptide bond