Description
Recombinant 2019-nCoV Spike protein S1 subunit, RBD (L452R T478K)(319-541) was expressed in CHO cells using a C-terminal his tag. The gene accession number is MN908947.
The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein recognizes the host ACE2 receptor and is a major determinant of viral entry and neutralization (1). SARS-CoV-2 variant B.1.617.2 (delta) carrying mutations L452R, T478K in the RBD has been reported to severely affect regions of India (2). This variant has been shown to have higher transmissibility and pathogenicity in hamster model, thus raising concerns about the neutralizing capability of vaccines. As new variants emerge, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (3)