Description
Short Description: Full-length recombinant p53 protein was expressed in a baculovirus system with a His-Tag and purified by an affinity column in combination with FPLC chromatography. We also offers Recombinant p53 protein that was expressed in E Coli. p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFkB (NFkB p50 and >NFkB p65), both of which serve to inhibit apoptosis. Recombinant p53 is used in TransAM, DNA-protein (gelshift) and protein-protein interaction assays