Description
Protease K is a kind of high activity protease of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on. Enzyme activity, > 30 U/mg. At 37 degree C, the amount of Protease K that can produce amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive in one minute with hemoglobin as the substrate is defined as a unit of protease K activity. The effective pH range of Protease K is pH4.0~12.5, and the optimal pH range is pH7.5~8.0. The optimum reaction temperature of protease K is 65 degree C, but at 65 degree C or higher, Protease K can also degrade rapidly. It is suggested that the optimum reaction temperature is 50~55 degree C