Description
Recombinant full-length human AMPK (combination of A2/B2/G3 subunits) was expressed by baculovirus in Sf9 insect cells using the N-terminal GST and C-terminal His tags. The gene accession numbers for the three subunits (A2/B2/G3) are NM_006252, NM_005399 and NM_017431.
AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes (1). AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization (2)