Description
Short Description: Recombinant NFkB p50 protein was expressed from a full-length cDNA clone in E Coli (accession number P19838). Protein was purified by affinity chromatography, followed by gel filtration. NFkB p50, is a subunit of the NFkB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains; they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFkB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IkB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IkB proteins. This releases NFkB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes. Recombinant NFkB p50 is suitable for both gelshift and TransAM assays