Description
Short Description: Recombinant Mononucleosomes (H3K27ac) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NP_003503.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3.1 that includes amino acids 1-136 (end)(accession number NP_003520.1) with acetylation at lysine 27, and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). Histone H2A, H2B and H4 were expressed in E Coli. H3K27ac was synthesized completely. Histone H3K27ac corresponds to the native histone sequence and does not contain any amino acid substitutions or residue analogs. The molecular weight of histone octamer is ~108 kDa. Nucleosomes comprise the smallest subunit of chromatin; they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery