Description
Short Description: Recombinant Mononucleosomes H3K9me3 (EPL) consist of 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NP_003503.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3.2 that includes amino acids 1-136 (end)(accession number NP_066403.2) with trimethylation at lysine 9, and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). All of these histones were expressed in E Coli. The molecular weight of histone octamer is ~108 kDa. H3K9me3 (Histone H3 trimethyl Lys9) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E Coli and purifed using FPLC. The purified protein is subsequently ligated to an N-terminal histone tail peptide containing trimethyl lysine 9 via a native peptide bond