Description
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Combined with CDC42 (#MBS515426) at a ratio of 1:4 (PAK1:CDC42), in vitro. The PAK1 gene accession, number is NM_002576.
PAK1 is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, CDC42 and RAC. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3 (1). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix (2)