Description
Short Description: Recombinant TRb1 protein is isolated from an E Coli strain that carries the coding sequence of the human TRb1 isoform under the control of a T7 promoter (accession number NM 000461) and purified by a combination of affinity and gel filtration chromatography. TRa1 protein has an N-terminal His-Tag and is purified free of contaminating proteases, endonucleases and exonucleases, and is visually determined to be >>=95% pure on an SDS-polyacrylamide gel. Recombinant TRb1 is for in vitro transcription, chromatin remodeling and protein interaction assays