Description
Short Description: Recombinant ER is specific for the ER alpha subunit and was expressed from full-length (accession number NM 000125) with an N-terminal His-Tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity. Estrogen receptors (ER) are nuclear hormone receptors that mediate the actions of the endogenous steroid hormone, 17b-estradiol (E2). ER is synthesized as two protein forms, ERa and ERb, which can bind to the estrogen receptor element (ERE) as homo- or heterodimers. Cell proliferation and differentiation triggered by ER activation has been heavily studied in bone-wasting and breast cancer treatments. Estrogen receptors are necessary for sexual development and reproductive function. Recombinant ER is suitable for DNA-protein (gelshift) and protein-protein interaction assays