Description
SARS-CoV2 virus receptor binding domain (318 to 542) within S1 of Spike protein was cloned, expressed and purified from Baculovirus expression system. This protein contains SARS-CoV2 leader sequence and RBD 318-542 amino acids and a 6 x His tag at its C terminal, migrated about 83kDa forming trimer structure on SDS-PAGE, it was recognized by specific human IgG from Pfizer SARS-CoV2 RNA vaccinated blood and the recovery antibody from SARS-CoV2 infected patients on Western blot.
Like the real virus forming trimer structure, recombinant SARS-CoV2 RBD or spike protein may be present as monomer, dimer, or trimer. On our Western blot, our recombinant SARS-CoV2 RBD trimer showed the equal intensity on Western blot as RBD monomer from the outsource product