Description
Recombinant human fusion KIF5B (1-921a.a., exons1-24) -ALK (1058a.a.-end, exons20-29) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number of KIF5B is NM_004521 and ALK is NM_004304.
Anaplastic large cell lymphomas are associated with chromosomal aberrations involving the anaplastic lymphoma kinase (ALK) gene, resulting in the expression of novel chimeric ALK proteins with transforming properties. Many chromosomal rearrangements leading to enhanced ALK activity have been described and are implicated in a number of cancer types (1). A fusion protein of kinesin family member 5B (KIF5B) exon 15 to ALK exon 20 has been reported in a primary lung adenocarcinoma (2). Overexpression of KIF5B-ALK increased cell proliferation, migration and invation via activating the STAT3 and PKB (3)