Description
Recombinant human FLT3-ITD-NPOS (571-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_004119. This FLT3-ITD mutant has 28 amino acids "CSSDNEYFYVDFREYEYDLKWEFPRENL" repeated between the sites L610 and E611 within the juxtamembrane domain.
Fms-related tyrosine kinase 3 (FLT3) is a gene that encodes for a tyrosine kinase that activates pathways in hematopoietic cells important in cellular proliferation. FLT3 is frequently mutated in acute myeloid leukemia (AML), myelodysplastic syndromes, other hematologic malignancies, and colorectal cancer. FLT3 mutations are observed in 24.3% of AML, of which internal tandem duplications (ITD) are the most frequent. FLT3 ITD occurs when sequences of less than ten to several hundred bases in length are repeated within the juxtamembrane domain are always "in-frame"