Description
CFP-10, a secreted protein, acts as a strong host (human) T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA; native protein binds poorly to artificial liposomes in the absence or presence of EsxA. EsxA and EsxA-EsxB are cytotoxic to pneumocytes. EsxB (and EsxA-EsxB but not EsxA alone) activates human neutrophils; EsxB transiently induces host (human) intracellular Ca2+ mobility in a dose-dependent manner, monocytes and lymphocytes do not respond. Neutrophils respond to EsxB by chemotaxis and primed neutrophils treated with EsxB produce reactive oxygen species (ROS); Ca2+ release and the ROS burst via are induced by an unidentified G-protein coupled receptor. May help regulate assembly and function of the type VII secretion system (T7SS).
Recombinant TB antigen CFP-10 is produced by our E Coli expression system and the target gene encoding Met1-Phe100 is expressed with a 6His tag at the N-terminus. Accession: P9WNK5 (Known as): ESAT-6-like protein EsxB; 10kDa culture filtrate antigen CFP-10; CFP-10 Mol Mass: 13kDa, AP Mol Mass: 14kDa, reducing conditions