Description
Short Description: The peptide corresponding to amino acids 2551-2715 of the MLL4 protein (accession number NP_055542.1) was expressed in E Coli. The peptide contains the SET domain of MLL2 that is responsible for methyltransferase activity. Recombinant MLL4-SET contains an N-terminal GST-Tag with a molecular weight of 45.2 kDa. Myeloid/lymphoid or mixed-lineage leukemia 4 (MLL4) is a Trithorax-group protein that function collectively to promote gene expression. MLL4 is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL4 plays a central role in b-globin locus transcription regulation by being recruited by Nfe2. MLL4 is also required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that precedes resumption of meiosis, oocyte survival and normal zygotic genome activation. MLL4 may also be involved in human cancer as its expression has been shown to be upregulated in solid tumor cell lines. Recombinant MLL4-SET protein can be used for enzyme kinetics and inhibitor screening studies