Description
PSMA1/FOLH1, as known as glutamate carboxypeptidase 2 (GCPII), is a single pass type 2 membrane protein which belongs to the peptidase M28 family. It is a zinc metalloenzyme that resides in membranes and catalyzes the hydrolysis of N-acetylaspartylglutamate (NAAG) to glutamate and N-acetylaspartate (NAA). This protein is most highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, live, stomach, small intestine colon, and the capillary endothelium of a variety of tumors. Thus, it shows a promising role in directed imaging and therapy of recurrent of metastatic disease. Recombinant human PSMA1/FOLH1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques