Description
Recombinant tag-free Alicyclobacillus acidiphilus AapCas12b was expressed in E Coli cells. The protein accession number is WP_067623834.
CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). AapCas12b belongs to the type V CRISPR effector, CRISPR-Cas12b/C2c1, and due to its thermostability, it can be used in a wide range of biomedical applications including loop-mediated isothermal amplification (LAMP), mammalian genome editing, and gene activation (4)