Description
Recombinant streptococcus HtrA (S234A)(33-393) protein was expressed in bacterial cells using an N-terminal His-tag. The gene is cloned from bacterial strain ATCC 700669 and the protein accession number is B8ZQD0. This protein, has a serine protease dead mutation.
HtrA (also designated as DegP) is a trypsin-like serine protease which contains PDZ domain and involves in heat-shock response, chaperone function, and apoptosis (1). Analysis of strains with the htrA (S234A) catalytic site mutation showed that the proteolytic activity of HtrA selectively repressed competence when translational fidelity was high but not when accuracy was low (3)